诱生型一氧化氮合成酶基因在隐睾中的表达及意义

Expression of induce nitric oxide synthase in experimental cryptorchidism

目的 :探讨隐睾中诱生型一氧化氮合成酶 (i NOS)基因表达与生殖细胞凋亡的关系。方法 :选取日龄为2 2 d的 SD雄性大鼠 2 0只 ,建立单侧隐睾模型 ,用生物素 - d U TP/酶标亲和素测定法检测睾丸生殖细胞凋亡 ,用免疫组织化学 SP法检测 i NOS基因表达。结果 :隐睾生殖细胞凋亡指数在术后 7d内随时间的延长而增加 ;从术后第 4天起 ,与对侧正常睾丸相比 ,隐睾发生凋亡的生殖细胞显著增加 (P <0 .0 1) ;在正常的睾丸组织中 ,i NOS定位于间质细胞、支持细胞和初级精母细胞的胞浆中 ,在隐睾精曲小管脱落的细胞胞浆中 ,i NOS免疫染色加强。结论 :生殖细胞凋亡增加 ,i

Purpose:To study the expression of induced nitric oxide synthase(iNOS) and testicular germ cell apoptosis in experimental cryptorchidism.Methods:Immature rats(22 day-old, Sprague Dawley) were subjected to unilateral cryptorchidism; Using terminal-deoxynucleotidy1 transferase mediated dUTP nick end labeling(TUNEL)for the detection of germ cell apoptosis; Using immunohistochemical SP method for the detection of expression of iNOS.Results:①After operation, the number of apoptotic germ cells in the cryptorchidism tissue increased when the time prolonged within 7 days.②The number of apoptotic germ cells in the cryptorchidism tissue increased significantly as compared to the contralateral control testis of the same animal from the 4th day to the 7th day after operation.③In control testis, iNOS immunostaining was Localized to the cytoplasm of Leydig cell, sertoli cells and primary spermatocytes; Additional intense immunostaining was detected in the cytoplasm of sloughed germ cells of tubules in the cryptorchidism.Conclusions:Expression of iNOS is believed to be one of the causes that increase the number of apoptotic germ cells in the cryptorchidism.