更昔洛韦的体外抗乙肝病毒活性

Anti-hepatitis B virus effect of gancicloriv(GCV) in vitro

目的 :评价更昔洛韦 (GCV)体外抗乙肝病毒 (HBV )活性。方法 :应用HBVDNA转染的人肝癌细胞株 (HepG22 .2 .15 ) ,进行GCV体外抗HBV活性的研究。结果 :GCV能明显减少HepG2 2 .2 .15细胞培养上清液中HBsAg、HBVDNA的产生。Southern印迹法分析 :GCV能显著抑制HBV复制中间体 (包括共价闭合环状态DNA) ,而同时该药物对细胞形态、细胞计数及总细胞DNA无明显的影响。结论 :GCV作为新的抗HBV药物 ,具有抑制HBV复制的活性 ,而细胞毒性作用不明显 ,值得临床上进一步推广应用。

OBJECTIVE:To study the inhibitory effect of gancilovir(GCV) on hepatitis B virus(HBV) replication in vitro .METHODS:To evaluate inhibitory effect of GCV with HBV transfected HepG 2 2.2.15 cell line in vitro .RESULTS:GCV could remarkably reduce the secret of HBsAg and HBV DNA in HepG 2 2.2.15 cell culture medium.Southern blot analysis demonstrated that GCV could remarkably inhibit HBV replicative intermediates including covalentely closed circular DNA. GCV and not significantly affect to cell morphology,cell counting and total cellular DNA.These results indicated that GCV can inhibit HBV replication in vitro ;meanwhile,the toxic effect to cells is not significant.CONCLUSIONS:GCV,as a novel,broad spectrum nucleotide analogue has anti HBV activity,can be used further in clinical study. [