基于硼酸盐聚合物绑定的SPR葡萄糖测量方法

A Surface Plasmon Resonance Glucose Measurement Method Based on Borate Polymer Binding

微创血糖检测技术通过测量人体组织液中葡萄糖浓度来预测人体血液中葡萄糖浓度，从而指导胰岛素的注入来控制和治疗糖尿病．由于透皮抽取得到的组织液体积微小且成分复杂，要求高测量精度和排除其他物质的干扰，提出一种基于硼酸盐聚合物绑定的表面等离子共振（surfaceplasmonresonance，SPR）葡萄糖浓度测量方法．表面等离子共振测量具有很高的测量精度，硼酸盐聚合物PAA．ran—PAAPBA对葡萄糖分子具有特异性吸附作用，而且性质稳定、制作简单．通过层层自组装技术将其绑定在SPR传感器金膜表面，可实现特异性检测并提高测量灵敏度．测量结果显示葡萄糖浓度在2—10mg／dL范围内二次曲线拟合度为0．90177，25～1000mg／dL范围内拟合度为0．99509，而且队A．ran．PAAPBA和葡萄糖分子的动态解离情况良好，满足连续血糖测量的需求，

The minimally-invasive human blood glucose detection technology is to measure the glucose concentration in interstitial fluid to calculate human blood glucose concentration and then guide the injection of insulin to control and treat diabetes. As the interstitial fluid obtained by transdermal extraction is tiny and complex, a surface plasmon resonance （SPR） glucose measurement method based on PAA-ran-PAAPBA polymer binding was proposed. The SPR measurement has high concentration measurement accuracy and the polymer PAA-ran-PAAPBA is chemically stable and easy to synthesize which can specifically absorb glucose molecule. The polymer was immobilized on the gold film of SPR sensor by layer-by-layer technique to realize the specific detection and thus improve the measurement sensitiv- ity. The experimental results show that the fitting degree is 0.901 77 when the glucose concentration level is between 2 mg/dL and 10 mg/dL and 0.995 09 between 25 mg/dL and I 000 mg/dL, and the dynamic dissociation process of PAA-ran-PAAPBA and glucose molecules is good, which meets the requirement of human blood glucose measure- ment.