基膜聚糖基因在侵袭转移子宫内膜癌组织中的表达

Expression of Lumican in Invasive and Metastatic Endometrial Carcinoma Tissues

目的探讨不同侵袭、转移性的子宫内膜癌组织中基膜聚糖基因(Lumican)的表达差异性。方法选取48例子宫内膜癌以及正常内膜组织,采用半定量RT-PCR及Western blot分别检测LumicanmRNA及蛋白表达水平,SPSS 13.0软件包统计分析Lumican在不同侵袭、转移性的子宫内膜癌组织中的表达差异性。结果 Lumican mRNA及蛋白在内膜癌组织中表达明显低于正常内膜组织[(0.54±0.042)vs.(0.87±0.035);(0.42±0.026)vs.(0.83±0.037)],且表达水平间的差异有统计学意义(P<0.05)。48例子宫内膜癌组织中,子宫浅肌层侵犯组(22例)、子宫深肌层侵犯组(11例)或宫颈及宫旁侵犯组(11例)、淋巴结转移组(4例)四组之间组织中Lumican mRNA及蛋白表达差异有统计学意义(P<0.05);子宫深肌层侵犯与宫颈及宫旁侵犯组之间Lumican表达差异无统计学意义(P>0.05)。结论 Lumican在子宫内膜癌组织中表达水平下调;Lumican的表达与子宫内膜癌组织侵袭、转移性负相关。Lumican的低表达可能参与子宫内膜癌的侵袭、转移恶性生物学行为过程。

Objective To explore the expression profiles of Lumican in invasive and metastatic endometrial carcinoma.Methods Forty-eight cases of endometrial carcinoma and paired normal endometrium tissues were applied for detecting the expression of Lumican.The mRNA and protein expression profiles of Lumican were analysed by RT-PCR and Western blot,respectively.SPSS13.0 statistical software was adopted to analyze the expression difference of Lumican in endometrial carcinoma.Results Compared with the normal endometrium tissues,mRNA and protein expression of Lumican were down-regulated in endometrial carcinoma tissues [（0.54±0.042）vs.（0.87±0.035）;（0.42±0.026） vs.（0.83±0.037）],respectively（P〈0.05）.It was showed evidently difference of Lumican expression in 48 cases of endometrial carcinoma,including superficial invasive group（22 cases）,deep invasive group（11 cases） /cervix invasive group（11 cases） and lymph metastatic group（4 cases）.Conclusion Lumican was downregulated in endometrial carcinoma,Lumican expression was negatively related to the invasion and metastasis of endometrial carcinoma.Low expression of Lumican may be involved in the malignant biological behavior of invasion and metastasis in the endometrial carcinoma.