新型维甲酸衍生物ATPR对胶质瘤U87细胞增殖和分化的影响

Effects of a novel retinoic acid derivative ATPR on proliferation and differentiation of human glioma cell line U87

目的:探讨新型全反式维甲酸衍生物4氨基-2三氟甲基苯基维甲酸酯(4-amino-2-triluoromethyl-phenyl retinate,ATPR)对人脑胶质瘤U87细胞增殖及分化的影响。方法:不同浓度的ATPR作用U87细胞后,分别采用细胞计数试剂盒-8(cell counting kit-8,CCK-8)法及平板克隆形成实验检测其对细胞增殖的影响;光学显微镜和电子显微镜下观察细胞形态的改变;FCM法检测对细胞周期分布的影响;蛋白质印迹法检测分化指标胶质纤维酸性蛋白(glial ibrillary acidic protein,GFAP)的表达。结果:ATPR对U87细胞的增殖抑制作用呈浓度相关性,单细胞克隆形成率下降,细胞形态趋于成熟;FCM法检测结果显示,G0/G1期细胞所占比例增加、S期细胞所占比例减少,细胞呈G0/G1期阻滞;蛋白质印迹法检测结果显示,GFAP蛋白表达量上升。结论:ATPR对人脑胶质瘤U87细胞有一定增殖抑制作用并诱导其分化。

Objective： To explore the effect of a novel retinoic acid derivative ATPR （4-amino-2trifluoromethyl-phenyl retinate） on proliferation and differentiation of U87 glioma cells in vitro.Methods： After treatment with ATPR at different concentrations,the proliferation of U87 cells was evaluated by CCK-8 （cell counting kit-8） and colony formation assay.The change in morphology of U87 cells was observed under a light microscope and an electron microscope.The distribution of cell cycle was examined by flow cytomentry.The expression of glioma differentiation marker GFAP （glial fibrillary acidic protein） was detected by Western blotting.Results： After treatment with ATPR,the proliferation was inhibited in a dose-relevant manner.The colony formation rate of single cells was declined,the cell morphology tended to be more mature,the expression of GFAP protein was increased,and the cell cycle progression was blocked in G 0 /G 1 phase.Conclusion： ATPR can inhibite the proliferation of human glioma U87 cells and induce cell differentiation.