垂体瘤转化基因在急性淋巴细胞白血病患者中表达的研究

Expression of Pituitary Tumor Transforming Gene in Patients with Acute Lymphoblastic Leukemia

本研究旨在探索垂体瘤转化基因(pituitary tumor-transforming gene,PTTG)在急性淋巴细胞白血病(acutelymphoblastic leukemia,ALL)患者中的表达及其与ALL发病机理的关系,同时探讨PTTG在Ph1染色体阳性和阴性ALL中的表达是否存在差异性。采用实时荧光定量PCR方法检测28例ALL患者及28例正常对照骨髓中PT-TG的表达水平。结果表明:ALL患者PTTG的表达水平(1.9428E5±1.8372E5)明显高于正常对照组(4.5766E3±1.1817E3)(P<0.05),一个周期诱导缓解治疗后达完全缓解(complete remission,CR)患者中PTTG的初始表达水平低于未缓解(non-complete remission,non-CR)患者;Ph1染色体阳性ALL(Ph1+ALL)患者中PTTG的初始表达水平高于Ph1染色体阴性ALL(Ph1-ALL)患者。结论:PTTG的过度表达可能和ALL的发生发展有关,可能与Ph1染色体阳性ALL的发生关系更加密切,这为ALL的发病机制及其基因靶向治疗的研究提供了新的思路。

The aim of this study was to explore the expression of pituitary tumor-transforming gene（PTTG） in acute lymphoblastic leukemia（ALL）and its relationship with the pathogenesis of ALL,as well as study the difference of the PTTG expression in ALL patients with Ph1 chromosome and without Ph1 chromosome.The mRNA expressions of PTTG in bone marrow from 28 patients with ALL and 28 normal controls were quantitatively detected by real-time quantitative polymerase chain reaction（real-time PCR）.The results indicated that the expression of PTTG mRNA was significantly higher in ALL patients（1.9428E5±1.8372E5） than that in normal controls（4.5766E3±1.1817E3）（P〈0.05）.The expression of PTTG mRNA was higher in Ph1 chromosome positive patients.The initial expression of PTTG mRNA was lower in patients achieved complete remission than that in patients with non-complete remission.It is concluded that the overexpression of PTTG may be related to the progression and genesis of ALL.Overexpression of PTTG may be intimately related to the progression and genesis of Ph1 chromosome positive ALL.It provides a new ideas to research the pathogenesis and genic target treatment of ALL.