FoxO3a在急性髓系白血病中的表达及其临床意义

Expression of FoxO3a in Patients with Acute Myeloid Leukemia and Its Clinical Significance

本研究旨在探讨叉头框蛋白O3a(forkhead box protein O3a,FoxO3a)在急性髓系白血病(acute myeloidleukemia,AML)中的表达及其临床意义。收集44例急性髓系白血病初诊患者骨髓标本,5例正常人骨髓作为对照,应用Western blot方法检测骨髓细胞FoxO3a蛋白的表达,其中14例患者在完全缓解后复测骨髓FoxO3a的表达。结果表明:AML患者骨髓细胞FoxO3a蛋白表达上调,平均表达量(灰度值)为0.43±0.19,显著高于正常对照组0.19±0.06(P<0.001);化疗缓解后FoxO3a蛋白水平下降,与正常对照组比较无显著性差异(P>0.10)。相关分析显示,FoxO3a蛋白表达与AML患者外周血白细胞数呈正相关(r=0.48,P=0.012);尽管FoxO3a表达量对缓解率无明显影响,但高表达者缓解时间短。结论:FoxO3a在AML中可能是一种原癌蛋白,初诊时高表达者预后差。

This study was aimed to investigate the expression and clinical significance of forkhead box protein O3a（FoxO3a） in the patients with acute myeloid leukemia（AML）.Western blot was used to detect the FoxO3a protein expression in bone marrow samples from 44 newly diagnosed AML patients and 5 healthy donors.Additionally,14 patients＇ samples were reevaluated when they got complete remission（CR）.The results showed that FoxO3a expression（FoxO3a/β-actin 0.43±0.19） in newly diagnosed AML patients was much higher than that in healthy donors（FoxO3a/β-actin 0.19±0.06）（P〈0.001）.The FoxO3a level was down-regulated when CR was got and there was not significant difference between patients in CR and healthy donors（P〈0.10）.The correlation analysis showed that the level of FoxO3a expression positively correlated with the white blood cell count of AML patients at the time of diagnosis.Although FoxO3a expression did not positively correlate with the CR rate,the higher FoxO3a expression in AML patients showed a shorter remission duration.It is concluded that FoxO3a may be a oncoprotein in AML,and the high FoxO3a expression is associated with poor prognosis.