Th22细胞及其细胞因子在急性淋巴细胞白血病表达水平及其意义分析

Expression Level of Th22 Cells and Its Cytokines in Patients with Acute Lymphoblastic Leukemia and Its Significance

本研究分析了Th22细胞及其细胞因子在急性淋巴细胞白血病(ALL)中的表达水平及其意义。选取确诊的ALL患者48例。根据治疗情况,将患者分为初诊未治组26例和完全缓解组22例。采用流式细胞术(FCM)检测Th22细胞情况,ELISA法检测血清IL-22、IL-6、TNF-α和TGF-β表达水平,半定量RT-PCR方法检测IL-22 mRNA的表达水平;同时,选取健康体检者30例为对照组,比较3组患者相关参数的差异。结果表明,初诊未治组和完全缓解组患者的Th22细胞水平,IL-22、IL-6、TNF-α、IL-22 mRNA表达水平均显著低于对照组,TGF-β表达水平显著高于对照组(P<0.05)。初诊未治组患者的Th22细胞水平,IL-22、IL-6、TNF-α、IL-22 mRNA表达水平均显著低于完全缓解组,TGF-β表达水平显著高于完全缓解组。初诊未治患者IL-22表达水平与IL-6、TNF-α表达呈正相关,与TGF-β表达呈负相关。结论:ALL患者Th22细胞数量减少,IL-22表达水平下降,可能与ALL发生有关。Th22细胞水平降低是ALL发生的危险因素。

This study was purposed to analyze the expression level of Th22 cells and their cytokines in patients with acute lymphoblastic leukemia（ALL） and evaluate its significance.Forty-eight patients with ALL were selected.According to the treatment,all patients were divided into the newly diagnosed group（n=26） and complete remission（CR） group（n=22）.The proportion of Th22 cells in peripheral blood was detected by flow cytometry（FCM）.The expression levels of cytokines IL-22,IL-6,TNF-α and TGF-β in peripheral blood were measured by ELISA.The expression level of IL-22 mRNA in peripheral blood mononuclear cells was examined by semi-quantitative-reverse transcription PCR（RT-PCR）.Meanwhile,30 healthy individuals were selected as a control group.The parameters of the 3 groups were compared.The results showed that the percentage of Th22 cells and the expression levels of IL-22,IL-6,TNF-α and IL-22 mRNA in newly diagnosed group and the CR group were significantly lower than that in control group,the expression level of TGF-β in above mentioned two group was obviously higher than that in control group（P〈0.05）.The percentage of Th22 cells and the expression levels of IL-22,IL-6,TNF-α and IL-22 mRNA in newly diagnosed group were evidently lower than that in CR group（P〈0.05）,but the expression level of TGF-β in newly diagnosed group obviously higher than that in CR group.The expression level of IL-22 in newly diagnosed group was positively related with expression level of IL-6 and TNF-α,but it was negatively related with expression level of TGF-β.It is concluded that the decreasing of Th22 cells and down-regulation of IL-22 expression level may be related with pathogenesis of ALL,the decreassing of Th22 cells is risk factor for ALL.