期刊文献+

Th22细胞及其细胞因子在急性淋巴细胞白血病表达水平及其意义分析 被引量:4

Expression Level of Th22 Cells and Its Cytokines in Patients with Acute Lymphoblastic Leukemia and Its Significance
下载PDF
导出
摘要 本研究分析了Th22细胞及其细胞因子在急性淋巴细胞白血病(ALL)中的表达水平及其意义。选取确诊的ALL患者48例。根据治疗情况,将患者分为初诊未治组26例和完全缓解组22例。采用流式细胞术(FCM)检测Th22细胞情况,ELISA法检测血清IL-22、IL-6、TNF-α和TGF-β表达水平,半定量RT-PCR方法检测IL-22 mRNA的表达水平;同时,选取健康体检者30例为对照组,比较3组患者相关参数的差异。结果表明,初诊未治组和完全缓解组患者的Th22细胞水平,IL-22、IL-6、TNF-α、IL-22 mRNA表达水平均显著低于对照组,TGF-β表达水平显著高于对照组(P<0.05)。初诊未治组患者的Th22细胞水平,IL-22、IL-6、TNF-α、IL-22 mRNA表达水平均显著低于完全缓解组,TGF-β表达水平显著高于完全缓解组。初诊未治患者IL-22表达水平与IL-6、TNF-α表达呈正相关,与TGF-β表达呈负相关。结论:ALL患者Th22细胞数量减少,IL-22表达水平下降,可能与ALL发生有关。Th22细胞水平降低是ALL发生的危险因素。 This study was purposed to analyze the expression level of Th22 cells and their cytokines in patients with acute lymphoblastic leukemia(ALL) and evaluate its significance.Forty-eight patients with ALL were selected.According to the treatment,all patients were divided into the newly diagnosed group(n=26) and complete remission(CR) group(n=22).The proportion of Th22 cells in peripheral blood was detected by flow cytometry(FCM).The expression levels of cytokines IL-22,IL-6,TNF-α and TGF-β in peripheral blood were measured by ELISA.The expression level of IL-22 mRNA in peripheral blood mononuclear cells was examined by semi-quantitative-reverse transcription PCR(RT-PCR).Meanwhile,30 healthy individuals were selected as a control group.The parameters of the 3 groups were compared.The results showed that the percentage of Th22 cells and the expression levels of IL-22,IL-6,TNF-α and IL-22 mRNA in newly diagnosed group and the CR group were significantly lower than that in control group,the expression level of TGF-β in above mentioned two group was obviously higher than that in control group(P〈0.05).The percentage of Th22 cells and the expression levels of IL-22,IL-6,TNF-α and IL-22 mRNA in newly diagnosed group were evidently lower than that in CR group(P〈0.05),but the expression level of TGF-β in newly diagnosed group obviously higher than that in CR group.The expression level of IL-22 in newly diagnosed group was positively related with expression level of IL-6 and TNF-α,but it was negatively related with expression level of TGF-β.It is concluded that the decreasing of Th22 cells and down-regulation of IL-22 expression level may be related with pathogenesis of ALL,the decreassing of Th22 cells is risk factor for ALL.
作者 陈荣华
出处 《中国实验血液学杂志》 CAS CSCD 北大核心 2013年第4期857-860,共4页 Journal of Experimental Hematology
关键词 TH22细胞 急性淋巴细胞白血病 细胞因子 白细胞介素22 Th22 cell acute lymphoblastic leukemia cytokine interleukin-22
  • 相关文献

参考文献11

二级参考文献109

  • 1Thomas DA, Faderl S, O' Brien S, et al. Chemoimmunotherapy with hyper-CVAD plus rituximab for the treatment of adult Burkitt and Burkitt-type lymphoma or acute lymphoblastic leukemia. Cancer, 2006, 106(7) :1569-1580.
  • 2Vitale A, Guarini A, Ariola C, et al. Adult T-cell acute lymphoblastic leukemia: biologic profile at presentation and correlation with response to induction treatment in patients enrolled in the GIMEMA LAL 0496 protocol. Blood, 2006, 107 (2) : 473-479.
  • 3Baak U, Gokbuget N, Orawa H, et al. Thymic adult T-cell acute lymphoblastie leukemia stratified in standard- and high-risk group by aberrant HOXllL2 expression: experience of the German multicenter ALL study group. Leukemia, 2008, 22 (6): 1154-1160.
  • 4Grabher C, von Boehmer H, Look AT. Notch 1 activation in the molecular pathogenesis of T-cell acute lymphoblastic leukaemia. Nat Rev Cancer, 2006, 6(5):347-359.
  • 5Pui CH, Relling MV, Downing JR. Acute lymphoblastie leukemia. N Engl J Med, 2004, 350(15) :1535-1548.
  • 6Moorman AV, Harrison CJ, Buck GAN, et al. Karyotype is an independent prognostic factor in adult acute lymphoblastic leukemia (ALL): analysis of cytogenetic data from patients treated on the Medical Research Council (MRC) UKALL XII/ Eastern Cooperative Oncology Group (ECOG) 2993 trial. Blood, 2007, 109(8): 3189-3197.
  • 7Pullarkat V, Slovak ML, Kopecky KJ, et al. Impact of cytogenetics on the outcome of adult acute lymphoblastic leukemia: results of Southwest Oncology Group 9400 study. Blood, 2008, 111(5): 2563-2572.
  • 8Bruggemann M, Raff T, Flohr T, et al. Clinical significance of minimal residual disease quantification in adult patients with standard-risk acute lymphoblastic leukemia. Blood, 2006, 107 (3): 1116-1123.
  • 9Szczepanski T. Why and how to quantify minimal residual disease in acute lymphoblastic leukemia. Leukemia, 2007, 21(4): 622- 626.
  • 10Gokbuget N, Raff R, Brugge-Mann M, et al. Risk/MRD adapted GMALL trials in adult ALL. Ann Hematol, 2004, 83( Suppl 1) : S129-S131.

共引文献65

同被引文献21

引证文献4

二级引证文献25

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部