IL-1β促进脐带间充质干细胞的造血支持作用

IL-1β Promotes the Hematopoietic Support of Human Umbilical Cord Mesenchymal Stem Cells

本研究旨在探讨IL-1β对人脐带间充质干细胞(hUC-MSC)的造血支持的影响。将分离得到的hUC-MSC以2×106接种于75 cm2培养瓶中,贴壁2 h后,加入10 ng/ml IL-1β培养36 h后收集培养上清和细胞。观察有/无IL-1β的条件培养液对CD34+细胞造血支持的影响;real time PCR检测加/不加IL-1βMSC的造血相关因子mRAN的变化;ELISA法检测造血相关因子的差异。结果表明,含有IL-1β的MSC条件培养液能明显增强CD34+细胞集落形成能力,且以粒系和粒-单核集落形成单位为主。IL-1β促进了MSC GM-CSF、G-CSF、IL-6的mRNA的表达,促进GM-CSF、G-CSF、IL-6蛋白自脐带MSC的分泌。结论:IL-1β能够增强MSC的造血支持能力,尤其是向髓系分化的能力。

This study was aimed to investigate the effect of IL-1β on hematopoietic support of human umbilical cord mesenchymal stem cells（hUC-MSC）.2×10^6 hUC-MSC were seeded in 75 cm2 flasks,after adherence to wall for 2 h,10 ng/ml IL-1β was added in hUC-MSC supernatant and cultured for 36 h,then the culture supernatants and cells were harvested.The effect of conditioned medium with/without IL-1β on CD34^＋ cell hematopoietic support was observed,mRNA expression changes of hUC-MSC cultured in medium with/without IL-1β were monitored by real time PCR,the differences in hematopoiesis-related factors were detected by ELISA.The results showed that the conditioned culture medium of hUC-MSC with IL-1β enhanced the ability to form colony of CD34^＋ cells,especially CFU-G and CFU-GM in vitro;IL-1β promoted the mRNA expression of GM-CSF,G-CSF,IL-6 on MSC;IL-1β also promoted the secretion of GM-CSF,G-CSF,and IL-6 protein from hUC-MSC.It is concluded that IL-1β enhances hematopoietic support capacity especially,capability of MSC to myeloid differentiation.