亚硝基谷胱甘肽对冷冻血小板膜糖蛋白分子的影响研究

Influence of S-nitrosoglutathione on the Membrane Glycoprotein of Frozen Platelets

本研究探讨亚硝基谷胱甘肽(S-nitrosoglutathione,GSNO)对冷冻血小板膜糖蛋白分子的影响。用流式细胞术对新鲜血小板、冷冻血小板及加入GSNO后的冷冻血小板的膜糖蛋白分子进行测定并进行比较。结果表明:GSNO明显降低了血小板的聚集率;PAC-1变化不显著;CD42b、CD62P变化在新鲜液态血小板组与冷冻血小板组、加有GSNO的冷冻血小板组差别显著;冷冻血小板组、加有GSNO的冷冻血小板组的血小板膜糖蛋白变化差别不显著。结论:GSNO抑制血小板的聚集,保持血小板的功能,可以用作冷冻保护剂。加入GSNO的冷冻血小板可能存在分子重排、结构上的变化及其它作用机制。

This study was purposed to explore the influence of S-nitrosoglutathione（GSNO） on membrane glycoprotein of frozen platelet.The levels of membrane glycoprotein on fresh liquid platelets,frozen platelets and frozen platelets with GSNO were measured by flow cytometry.The results showed that the GSNO obviously decreased platelet aggregation,the PAC-1 change in the three groups was not significant.The changes of CD42b and CD62P in fresh liquid platelet group,frozen platelet group and frozen platelets with GSNO were significant different.The change of membrane glycoprotein in above-menthioned three group was not significant.It is concluded that the GSNO inhibits platelet aggregation,maintains the function of platelets and may be used as a cryoprotectant.When frozen platelets were added with GSNO,the molecular rearrangement,structure change and other mechanism may occur in platelets.