尖端电喷雾萃取电离质谱技术诊断食管癌22例

Tip-spray ionization mass spectrometry for diagnosis of esophageal cancer:An analysis of 22 cases

目的:探讨尖端电喷雾萃取电离质谱技术(tipspray ionization mass spectrometry,Tip-sprayMS)对食管癌(esophageal cancer,EC)诊断的应用.方法:收集22例EC及配对的癌旁组织,应用Tip-spray-MS技术对组织样品进行快速分析,调节Tip-spray离子源为正离子模式,质量范围50-1000 Da,喷雾电压为3.5 kV,喷雾距离为2 cm,透镜电压为65.0 V,离子传输管温度为150℃.运用Matlab软件主成分分析(principal component analysis,PCA)方法对所获质谱指纹谱进行统计学分析.结果:EC与癌旁组织的质谱指纹谱图中相对丰度有明显差异的质荷比(m/z)为104、154、175、611、655、699、743、787、831等处,其中m/z为611、655、699处丰度差异最显著,其所对应的物质为卟啉,运用PCA方法能够良好地区分EC及癌旁组织.结论:Tip-spray-MS联合PCA能有效鉴别EC及癌旁正常组织,有助于EC的早期、快速诊断.

AIM： To discuss the application of tip-spray ion~ ization mass spectrometry （MS） in the diagnosis of esophageal cancer. METHODS： Tip-spray MS was used to analyze esophageal cancer tissue samples and matched tumor-adjacent esophageal tissue samples from 22 patients with esophageal cancer, Principal component analysis （PCA） was performed to process data from the MS fingerprint spectra RESULTS： Peptide peaks at m/z values of 104, 154, 175, 611, 655, 699, 743, 787 and 831 showed significantly different relative abundance. Peaks at m/z values of 611, 655 and 699, which cor- responded to porphyrin, had the most obvious difference. PCA could effectively distinguish esophageal cancer and tumor-adjacent normal tissue. CONCLUSION： Tip-spray MS combined with PCA can effectively distinguish esophageal cancer and tumor-adjacent normal tissue and therefore may contribute to the rapid diagnosis of esophageal cancer.