骨桥蛋白及其相关因子在乳腺癌组织中的表达及其临床意义

Expressions of Osteopontin and Related Factors in Breast Cancer Tissues and Its Clinical Significance

目的检测骨桥蛋白(OPN)、乳腺肿瘤激酶(Brk)和血管内皮生长因子(VEGF)在乳腺癌组织、癌旁近端非癌乳腺组织及癌旁远端正常乳腺组织中表达的差异并分析其临床意义。方法采用免疫组织化学方法检测40例乳腺癌组织、癌旁近端(2 cm)非癌乳腺组织及癌旁远端(>5 cm)正常乳腺组织中OPN、Brk和VEGF的表达情况。结果①OPN、Brk和VEGF蛋白在乳腺癌组织中的表达阳性率均明显高于其在癌旁近端非癌乳腺组织和癌旁远端正常乳腺组织中的表达(P<0.01),Brk在癌旁近端非癌乳腺组织中的表达明显高于其在癌旁远端正常乳腺组织中的表达(P<0.05),而OPN、VEGF在癌旁近端非癌乳腺组织中和癌旁远端正常乳腺组织中的表达差异均无统计学意义(P>0.05)。②OPN和Brk蛋白表达与患者年龄、肿瘤直径及组织学分级均无关(P>0.05),与淋巴结转移及TNM分期有关(P<0.05);VEGF与患者年龄及肿瘤直径无关(P>0.05),与组织学分级、淋巴结转移及TNM分期有关(P<0.05)。③在乳腺癌组织中OPN、Brk和VEGF表达之间两两呈正相关性(P<0.05),在癌旁近端非癌乳腺组织和癌旁远端正常乳腺组织中OPN、Brk和VEGF表达之间均无明显相关性(P>0.05)。结论位于同一信号传导通路上的OPN、Brk在癌旁远端正常乳腺组织、癌旁近端非癌乳腺组织和乳腺癌组织中的表达依次递增。OPN可能通过VEGF、Brk刺激内皮细胞黏附和迁移而加速血管修复,通过参与肿瘤血管生成与肿瘤的侵袭与转移有关。

Objective To detect the expressions of osteopontin（OPN）,breast tumor kinase（Brk）,and vascular endothelial growth factor（VEGF）in the breast cancer tissue,the adjacent（2 cm）normal breast tissue,and the distal（5 cm）normal breast tissue,and analyze their clinical significances.Method The immunohistochemical method was used to detect the expressions of OPN,Brk,and VEGF in the breast cancer tissue,the adjacent（2 cm）normal breast tissue,and the distal（5 cm）normal breast tissue from 40 cases of breast cancer.Results ① The expressions of OPN,Brk,and VEGF in the breast cancer tissue were significantly higher than those of the adjacent（2 cm）normal breast tissue and the distal（5 cm）normal breast tissue（P0.01）,the expression of Brk in the adjacent（2 cm）normal breast tissue was significantly higher than that of the distal（5 cm）normal breast tissue（P0.05）.② In the breast cancer tissue,the OPN and Brk protein expressions were not associated with age,tumor diameter,and histological grade（P0.05）,were associated with lymph node metastasis and TNM stage（P0.05）.The VEGF protein expression was not associated with age and tumor diameter（P0.05）,but was associated with histological grade,lymph node metastasis,and TNM staging（P0.05）.③ In the breast cancer tissue,OPN,Brk,and VEGF had positive correlation with each other（P0.05）,but not in the adjacent（2 cm）normal breast tissue and the distal（5 cm）normal breast tissue（P 0.05）.Conclusions The expressions of OPN and Brk from the same signal pathway increase by turns in the distal（5 cm） normal breast tissue,adjacent（2 cm）normal breast tissue,and breast cancer tissue.OPN induced the adhesion and migration of endothelial cells to accelerate vascular repair through VEGF and Brk has correlation with the progress of tumor invasion and metastasis through participating in tumor vascularization.