苦碟子注射液对大鼠随意皮瓣存活的影响

Effects of sowthistle-leaf Ixeris seedling injection on survival of random skin flap in rats

目的:观察苦碟子注射液研究苦碟子注射液对大鼠随意型皮瓣存活的影响对大鼠随意皮瓣成活的影响。方法:将2～3月龄重SD大鼠24只,采用随机抽签法分为2组(实验组和对照组,每组12只)。采用改良的大鼠随意型皮瓣制作方法造模,实验组每天腹腔注射苦碟子注射液5ml/kg,对照组每天腹腔注射生理盐水5ml/kg。术后第7天颈椎脱臼处死大鼠分别进行皮瓣存活面积比的检测,同时取皮瓣近中远端组织经组织染色切片后光镜下观察,免疫组化法检测血管内皮生长因子(VEGF)的表达。结果:术后7d,实验组皮瓣存活面积比为(70.432±3.867)%,显著高于对照组的(50.498±2.346)%(P<0.01);实验组皮瓣组织水肿、炎症细胞浸润情况比对照组明显减轻;切片观察出现较多新生血管,Ⅱ区新生血管密度((30.11±5.53)/mm2,与对照组(20.13±4.11))/mm2比较,差异有统计学意义(P<0.01),Ⅲ区坏死严重。实验组VEGF表达量为(4867.31±452.36),与对照组的VEGF表达量(2387.45±768.46)比较,差异有统计学意义(P<0.01)。结论:苦碟子注射液可以提高VEGF的表达量、促进毛细血管新生,减轻皮瓣炎性浸润,还可以明显提高大鼠随意型皮瓣的成活率,为临床皮瓣移植研究提供了新的思路。

Objective：To observe the effects of Ixeri sonhifolia injection on random skin flap survival in rats. Methods： Dorsal full thickness skin flap model were harvested from 24 Sprague-Dawley rats in 2 to 3 months old. Twelve rats in experi- mental group were injected intraperitoneally with 5 ml / kg Ixeri sonhifolia injection immediately after the operation ,the other rats in control group with an equal volume of saline. The rats were killed by cervical dislocation after 7 days＇ Ixeri sonhifolia in- jection. The area ratio of the survival tissue was measured at the 7th day ,the tissue samples from proximal,middle,and distal portions were stained by HE and sectioned for histological and image analysis. VEGF was detected by immunohistochemistry. Results：Seven days later,there was statistical significance between the percentage of the survival area of the flap between the experimental group （70.432 ±3.867）% and the control group （50.498±2.346）% （P0.05）. In the middle portion,edema and infiltration of tissue in the experimental group were reduced than those of the control group ,and new blood vessels in- creased in the experimental group （P0.05）. A statistical significance of the expression of VEGF was detected between experi- mental group （4867.31±452.36） and control group （2387.45±768.46）（P0.05）. Conclusion：Ixeri sonhifolia injection can promote the survival of random skin flap by increasing the quantity of capillary ,reducing inflammatory infiltration of Europhiles and increasing the expression of VEGF,which promote a new approaching for the transpanting of the random flap research.