川椒方对过敏性结膜炎小鼠IL4-JAK1-STAT6信号通路的影响

Effect of Chuanjiao formula on IL4-JAK1-STAT6 pathway of allergic conjunctivitis mice

目的观察川椒方对过敏性结膜炎小鼠IL 4-JAK1-STAT6信号通路的影响。方法用卵清蛋白免疫小鼠建立过敏性结膜炎模型,随机分为高、中、低剂量川椒方组、阳性药奥洛他定滴眼液组、模型组,另以健康小鼠作为空白对照组。川椒方各剂量组给予不同浓度的川椒方水煎剂灌胃,奥洛他定滴眼液组予盐酸奥洛他定滴眼液点眼,各组均治疗7 d。观察各组动物眼睑和结膜充血水肿、分泌物情况并做评分,应用酶联免疫吸附法(ELISA)测定血清IL 4、JAK1、STAT6含量。结果各剂量川椒方均能减轻动物眼睑和结膜水肿、充血。模型组的体征评分均值最高,明显高于空白对照组(P<0.01),川椒方各剂量组的体征评分均较模型组降低(P<0.01,P<0.05),各川椒方剂量组体征评分均值与奥洛他定滴眼液组结果接近(P>0.05)。川椒方高、中、低剂量组及奥洛他定滴眼液组的血清IL 4、JAK1、STAT6含量均低于模型组(P<0.01,P<0.05),川椒方高、中、低剂量组间差异无统计学意义(P>0.05)。结论川椒方能减轻过敏性结膜炎小鼠的眼部体征,并可能抑制IL4-JAK1-STAT6信号通路的激活。

OBJECTIVE To investigate the effect of Chuanjiao formula on IIA-JAK1-STAT6 pathway of al- lergic conjunctivitis mice. METHODS After induced allergic conjunctivitis by injecting ovalbumin, the mice were divided into blank group, model group, Olopatadine control group,high,medium and low dose Chuanjiao formula groups. Both Chuanjiao formula and Olopatadine were administrated for 7 days. To determine the efficacy, the changes of hyperemia and chemosis of eyelid and conjunctiva, exudation were scored. To study the changes of IIA- JAKI-STAT6 pathway, the concentration of L4, JAKland STAT6 in mice serum were measured by ELISA. RE- SULTS The hyperemia and chemosis of eyelid and conjunctiva,exudation in all Chuanjiao formula groups were re- lieved. The score signs of model group was the highest,compared with blank group （P〈0.01）. The scores of signs in all Chuanjiao formula groups decreased significantly compared with that of model group （P〈0.01, P〈0.05）, scores of signs approximated Olopatadine group （P〉0.05）. IL 4, JAK1, STAT6 in Chuanjiao formula groups and Olopatadine group were lower than that of model group （P〈0.01,P〈0.05）, and no statistical significance was found among Chuan- jiao formula groups （P〉0.05）. CONCLUSIONS Chuanjiao formula could relieve eye signs of allergic conjunctivitis mice and reduce the activated levels of IL4-JAK1-STAT6 signal pathway.