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盐胁迫诱导的TabZIP60转录因子的筛选与分析 被引量：1

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摘要 筛选与鉴定耐盐相关基因是培育抗盐品种的基础。植物转录因子在植物的生长发育及其对外界环境的反应中起着重要作用。利用小麦转录因子全长cDNA文库,运用Re-Northern和半定量RT-PCR相结合的方法,从小麦全长转录因子cDNA文库的526个转录因子进行了盐胁迫筛选,获得了与小麦抗盐相关的转录因子TabZIP60,分析这个转录因子的序列并进行了功能分析,获得如下结果:TabZIP60的长度是1 741 bp,编码338个氨基酸;TabZIP60受高盐诱导后表达量明显升高;GFP亚细胞定位表明该转录因子是一个核定位蛋白。
作者 刘骥 王燕 郭建华 刘晓兰 孔秀英
机构地区 齐齐哈尔大学食品与生物工程学院 中国农业科学院作物研究所
出处 《江苏农业科学》 北大核心 2013年第8期18-22,共5页 Jiangsu Agricultural Sciences
基金 引进国际先进农业科学技术计划("948"计划)(编号:2005-Z47)
关键词 TabZIP60 盐胁迫 转录因子 序列分析
分类号 Q785 [生物学—分子生物学]
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参考文献16

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二级参考文献35

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江苏农业科学
2013年 第8期

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