八种昆虫离体细胞系对灭多威农药的敏感性研究

Sensitivity Research of Eight Insect Cell Lines to Methomyl Insecticide

采用MTT法 ,研究了灭多威农药对八种昆虫离体细胞系敏感性的差异。结果显示 ,同一浓度处理下 ,不同细胞系细胞病变的程度不同 ,敏感性差异很大。LC50 值在 10 2 ～ 10 5μg/ml范围内变化 ,其中 ,白纹伊蚊细胞系是最敏感的 。

In this paper, a MTT-based semiautomated colormetric assay was used to study the sensitivity difference among eight cultured insect cell lines to Methomyl pesticide. The results showed at the same test concentration, the extent of cytopathic effect (CPE) among different cell lines was different ,the sensitivity difference was obvious. The LC 50 values varied from 10 2 to 10 5μg/ml, and C 7～10 cell line was more sensitive than other cell lines, while Px cell line was hardly sensitivity to Methomyl.