mRNA差异展示研究胃癌差异表达基因

Studies on Differentially Expressed Genes of Gastric Cancer by mRNA Differential Display.

以胃癌细胞株GC790 1与胃粘膜细胞株GES 1为对比材料 ,利用mRNA差异展示技术 ,研究胃粘膜至胃癌过程的差异表达基因 ,为建立胃癌预警系统打下基础 .获得 8个未知的与胃癌相关的cDNA ,命名为GCYS 1,2 ,3,4,5 ,6 ,7,2 0 ,完成其克隆及序列分析 ,RNA印迹证实GCYS 1至 7片段与GCYS 2 0基因在GC790 1细胞株中呈高表达 ,在GES 1细胞株中呈低表达 .此 8个序列在GenBank数据库中登录 ,接受号为AF0 5 416 2～AF0 5 6 16 8及AF2 19140 .

In order to establish warning system of gastric cancer, using cell line GC7901 and GES 1 as targets,8 cDNAs of differentially expressed genes between them were isolated by mRNA differential display,and then respectively named as GCYS 1 to GCYS 7,GCYS 20,and then were cloned into pGEM T vector. These cDNA fragments were up regulated in GC7901 and down regulated in GES 1 as shown by Northern blot.DNA sequencing demonstrated that all of them were novel.These sequences have been assigned the database accession numbers in GenBank as below:AF054162,AF054163,AF054164,AF054165,AF054166,AF054167,AF054168,AF219140.