摘要
我们用 12个随机扩增多态性DNA (RAPD)引物对来自不同品系的 4只鸡进行了RAPD分析 ,在扩增出的共 99条带中 ,表现多态性的带为 38条 ,占总带数的 38% .回收了 4个表现个体特异性的RAPD产物 ,当用鸡的基因组总DNA探针与它们杂交时 ,其中 3个表现阳性 ,说明RAPD方法扩增出的高变异产物含有重复序列 .用含重复序列的个体特异性RAPD产物作探针 ,与无关个体鸡基因组DNA的HaeⅢ酶切产物进行DNA印迹 ,获得了变异性较高的DNA指纹图谱 .因此 ,高变异的RAPD产物可以有效地用作DNA指纹探针 .
Four chickens, each from a different strain, were analyzed using randomly amplified polymorphic DNAs(RAPD) technique with 12 primers. 38 of 99(38%) detected fragments showed polymorphic. Four individual specific fragments were recovered and probed with labeled whole genome of chicken. Positive signal was detected in three of the fragments, suggesting that hypervariable fragments generated by RAPD contain repetitive sequences. Highly variable DNA fingerprints were generated when such repetitive sequence containing fragments were used as probe to hybridize to HaeⅢ digests of random chickensDNA. Therefore, RAPD products that are highly variable among individuals can be used as DNA fingerprinting probes.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2000年第4期426-429,共4页
Progress In Biochemistry and Biophysics
基金
国家自然科学基金资助项目!( 3 95 70 3 86)
