质谱在寡核苷酸药物质量控制中的应用

Application of Mass Spectrometry in Quality Control of Oligonucleotide Drugs

介绍了质谱在寡核苷酸质量控制方面的应用 .合成寡核苷酸及类似物作为反义治疗剂在病毒感染和一些癌症治疗方面有良好的前景 .寡核苷酸作为药物 ,其结构特性必须进行确证 .寡核苷酸浓度和纯度的分析可使用色谱或电泳技术 ,对寡核苷酸的碱基组成、序列、同一性 ,修饰基团 ,色谱或电泳分析方法无能为力 .质谱的高鉴别能力使其能有效、灵敏、快速和精确地确定寡核苷酸的这些特性 .

Synthetic oligonucleotides and their analogs have shown promising potential therapeutic agents in the treatment of viral infections and certain cancers. As drugs, the full characterization of oligonucleotides requires complete verification. Chromatographic or electrophoretic techniques can be used in analysis of concentration and purity of synthetic oligonucleotides, but the analytic methods have been limited in analysis of base composition, sequence , identity and modification of oligonucleotides. Ability of mass spectrometry to discriminate mass make MS effective, sensitive, rapid and accurate to determine these characterizations of oligonucleotides. application of mass spectrometry in quality control of synthetic oligonucleotides was introduced.