发情犬与未发情犬卵母细胞体外受精的研究

Stuadies on the Fertilization of Estrous Dog and Diestrous Dog Oocytes in Vitro

采集发情犬和未发情犬卵巢 ,回收大腔 (直径大于 2mm)、小腔 (直径 1～ 2mm)和不可见卵泡卵母细胞 ,进行体外受精 ,结果如下 :①发情组的单精受精率 (31 2 % ) ,原核形成率 (1 9 4% )高于未发情组。发情组的多精受精率 (1 2 6% )要低于未发情组。②直径 1～ 2mm、大于 2mm卵泡组单精受精率略高于不可见卵泡组。可见卵泡组的原核形成率高于不可见卵泡组。③发情犬卵泡直径大于 2mm组卵裂率高于发情犬和未发情犬不可见卵泡卵母细胞。

Received ovaries from estrous dogs and diestrous dogs. The oocytes derived from >2 mm and 1-2 mm antral follicles on estrous dogs and dietrous dogs. These oocytes were inseminated for 12h. The results were described as follows:\;①In the estrous group, the monospermy rate(31.2 %), pronuclei formation rate(19.4 %) were higher than that in the diestrous group and the polyspermy rate(12.6 %) was lower than that in the diestrous group. ②The monospermy rate of the antral follicles groups of 1-2 mm and >2 mm were higher than that of the invisible follicles group, and the pronuclei formation rate were higher than that of the invisible follicles group. ③The cleavage rate of >2 mm antral follicles was higher that of the oocytes derived from invisible follicles inestrous ovaries and diestrous ovaries. the clevage rates of oocytes derived from invisible follicles in estrous ovaries and in diestrous ovaries were no difference.