红莲型细胞质雄性不育水稻线粒体DNA的RFLP分析

RFLP Analysis for Mitochondria Genome of CMS Rice Honglian Type

应用RFLP技术 ,研究了红莲型不育系、保持系、杂种一代及野败型、马协型不育系的线粒体基因组。结果表明 ,红莲型不育系与保持系线粒体基因组之间在多个基因区域存在明显差异 ,为红莲型细胞质雄性不育分子机理研究提供了线索 ;红莲型不育系与野败型不育系的线粒体基因组之间存在显著差异 ,马协型不育系与野败型不育系的线粒体基因组之间存在一定差异 ,在分子水平揭示了不育胞质的多样性。

Restriction fragment length polymorphism (RFLP) was used to analyze rice mitochondrial genome.The results indicated obvious differences between mt genome of rice cytoplasmic male sterile line and its maintain line of Honglian type,which further clued CMS mechanism research.Mitochondrial genome difference between sterile line of Honglian type and Wild Abortive type was obvious,some difference between sterile Maxie and Wild Abortive was also detected,it offered molecular evidence for cytoplasmic heterogeneity.