泡状棘球蚴感染大鼠肝移植模型的建立

The building of the model of orthotopic liver transplantation in Echinococcus Mutilocularis （E. m） infected rats

目的研究泡状棘球蚴感染大鼠原位肝移植模型的手术技巧及术后并发症的预防与处理。方法50只BN大鼠作供体，50只泡状棘球蚴感染的Lewis大鼠为受体，通过“二袖套法”施行大鼠原位肝移植。用改进的二袖套法对大鼠行原位肝移植，移植中门静脉、肝下下腔静脉用袖套法进行吻合，肝上下腔静脉用缝合法吻合，胆道采用支架法进行胆道重建。结果建立大鼠原位肝移植模型共50例，术中未出现死亡，存活时间最短为5h，48h存活率为96％（48／50）。结论只有熟练地掌握手术技巧，细致耐心的操作，最大程度减少各种并发症的发生，才能得到良好的肝移植模型。

Objicetive To study the technique infected rats. Methods liver was established by Orthotopicl liver tran using ＂two-cuff meth and complication of orthotopicl liver transplantation in E. m splantation in 50 BN rats that accepted E. m. infected Lewis od＂. Totally rats were treated with liver transplantation by improved two-cuff method in the portal vein, hepatic inferior vena eava under the law in line with cuff, liv- er superior and inferior vena cava were sutured with suture line. Biliary tract reconstruction was completed in bile duct stent law. Results Orthotopic liver transplantation model was established in 50 rats with no mortality. The eean survival time was 5 h, the survival rate of post-operation 48 h was 96%. Conclusion Meticulous and skilled surgical technique is the key of successful operation. To operate patiently and care- fully and reduce various complications can get a good rat model of orthotopie liver transplantation.