倍频Nd:YAG激光对钝顶螺旋藻的诱变效应

Mutagenesis of Spirulina platensis by Nd:YAG Laser

利用倍频 Nd:YAG激光 (波长 53 2 nm,功率 50 0 m W,功率密度 1 60 m W/cm2 )诱变钝顶螺旋藻 ,辐照时间为 1 5min、1 0 min、5min通过测定藻丝形态参数、叶绿素 a、β 胡萝卜素、生长速度 ,比较倍频 Nd:YAG激光对钝顶螺旋藻生长的影响。实验结果表明 :与出发株相比 ,经倍频 Nd:YAG激光辐照后 ,藻丝形态发生变化 ,藻丝长、螺旋数、螺旋长变小 ;1 5min,1 0 min辐照组出现螺旋变松驰 ;1 0 min,5min辐照组促进藻的生长和叶绿素 a含量提高 ,使生长速度提高。三个诱变时间剂量都有利于 β 胡萝卡素积累 ,含量增幅最高达 2 2 .3 %。

Nd:YAG Laser(wavelength 532nm, power 500mW, power density 160mW/cm 2) was used irradiate Spirulina platensis, and with irradiate does of 15min, 10min, 5min. The followin parameters were used in assays:morphology, chorophyll a and β carotene. The results showed that the morphology parameters differenced after Nd:YAG mutagenesis. The filaments of the mutants showed shorter length and thread pitch and fewer numbers of the helix than of the initial stain, Also some variform filaments appeared. The does of 10min and 5min stimulated the growth and chlorophyll a of Sp platensis. Three irradiate does could enrich βcarotene, and the productivity of βcarotene was in creased most by 22.3%