白介素-2促进RC-4B/C垂体瘤细胞系增殖

Interleukin-2 stimulates the proliferation of cultured RC-4B/C pituitary adenoma cell line

采用细胞培养方法 ,以3 H TdR掺入率反映细胞增殖水平 ,研究了白介素 2 (IL 2 )对大鼠源的RC 4B/C垂体瘤细胞系增殖的影响 ,并初步分析了IL 2的作用机制。结果表明 :(1)IL 2 (10～ 10 0 0U/ml)剂量依赖性地显著提高RC 4B/C细胞3 H TdR掺入率。(2 )特异性酪氨酸蛋白激酶 (PTK)抑制剂tyrphostin (1μmol/L)可明显降低RC 4B/C细胞3 H TdR掺入率 ,并可部分阻断IL 2的促细胞增殖作用。(3)特异性蛋白激酶A (PKA)抑制剂H 9(1μmol/L)作用后 ,3 H TdR掺入率明显升高 ;H 9还可加强IL 2的促细胞增殖作用。(4)在本实验中未观察到抗雌激素tamoxifen (1μmol/L)对IL 2促RC 4B/C细胞增殖效应有明显影响。上述结果表明 ,IL 2参与调节垂体瘤细胞的增殖活动 ,并且IL 2的作用与PTK及PKA信号传导途径有关。

The aim of the present study was to investigate whether interleukin 2 (IL 2) is involved in the proliferation control of the cultured RC 4B/C cell, which is a derived pituitary adenoma cell line of the rat. The level of cell proliferation was estimated by assessing ~ 3H thymidine (~ 3H TdR) incorporation rate. IL 2 (10～1?000 U/ml) significantly stimulated ~ 3H TdR incorporation into the cell line in a dose dependent manner. Specific PTK inhibitor tyrphostin (1 μmol/L) suppressed RC 4B/C cell proliferation and blocked the effect of IL 2 on RC 4B/C cells. After the PKA signaling pathway was inhibited by specific PKA inhibitor H 9 (1 μmol/L), the proliferation rate of RC 4B/C cells increased significantly. H 9 also enhanced the stimulation of IL 2 on RC 4B/C cell growth. Anti estrogen tamoxifen (1 μmol/L) had no significant effect on the action of IL 2 on the proliferation of RC 4B/C cells. In conclusion, it is suggested that IL 2 modulates the proliferation of the cultured RC 4B/C pituitary adenoma cell line, and the action is closely related with the PTK and PKA signaling pathway.