碱性成纤维细胞生长因子增加大鼠血管一氧化氮及内皮素的生成

Basic fibroblast growth factor increases nitric oxide and endothelin production in rat aorta

研究碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)对自发性高血压大鼠 (SHR)和WKY大鼠血管一氧化氮 (NO)及内皮素生成的影响。取SHR和WKY大鼠主动脉制成血管薄片 ,以 10、10 0ng/mlbFGF(终浓度 )分别孵育 6h ,测定血管组织中一氧化氮合酶 (NOS)活性及孵育液中亚硝酸盐 (NO 2 )和内皮素含量。结果显示 :SHR主动脉组织NOS活性较WKY的低 17 6 % (P <0 0 1) ,血管薄片孵育液中NO 2 含量低 5 9 7% (P <0 0 1) ,内皮素浓度高 37 4% (P <0 0 1)。在低、高浓度bFGF刺激下 ,SHR主动脉组织NOS活性分别增高 2 9 7%和 5 9 6 %(均P <0 0 1) ,主动脉薄片孵育液中NO 2 含量分别增加 2 8 2 % (P <0 0 5 )和 70 5 % (P <0 0 1) ,主动脉内皮素的生成分别增加 2 4 1%和 44 5 % (均P <0 0 1) ;WKY大鼠主动脉组织NOS活性分别增高 2 4 4%和 5 3 7% (均P <0 0 1) ,主动脉薄片孵育液中NO 2 含量分别增加 18 8%和 2 5 9% (均P <0 0 1) ,主动脉内皮素的产生分别增加84 1%和 93 1% (均P <0 0 1)。结果表明 :bFGF对SHR和WKY大鼠主动脉NO及内皮素的产生具有调变作用。

The study was undertaken to investigate the effect of basic fibroblast growth factor (bFGF) on aortic production of nitric oxide (NO) and endothelin of aorta in spontaneously hypertensive rats (SHR) and WKY rats. Rat aortas were cut into vessel slices and incubated with 10 or 100 ng/ml bFGF for 6 hours. Nitric oxide synthase (NOS) activity in aortic slices and contents of NO 2 and endothelin in the medium were de^ter^mined. The results showed that NOS activity in aortic slices of SHR was 17 6% lower than that of WKY ( P <0 01). NO 2 and endothelin contents in the medium of SHR aortic slices were 59 7% lower and 37 4% higher than those of WKY aortic slices. Upon the exposure of low and high doses of bFGF, NOS activity in the aorta of SHR was increased by 29 7% and 59 6% (both P<0 01),respectively, while the NO 2 contents in the medium were increased respectively by 28 2% (P<0 05) and 70 5% (P<0 01). Aortic endothelin production was increased by 24 1% and 44 5%(both P<0 01) respectively while the NOS activity in the aorta of WKY was increased by 24 4% and 53 7% (both P<0 01). NO 2 contents in the medium were augmented by 18 8% (P<0 05)and 25 9% (P<0 01), respectively. Aortic endothelin production was increased by 84 1% and 93 1% (both P<0 01). It is concluded that bFGF may modulate the production of NO and endothelin in both SHR and WKY rats.