利用花色素苷合成调节基因C1-R 作为基因枪转化效果指示的研究

Estimation of Biolistic Transformation Effect by Transient Expression of C1-R Regulatory Genes of Anthocyanin Biosynthesis

报道了玉米花色素苷合成调节基因Ｃ１－Ｒ在小麦幼胚、玉米愈伤组织、水稻愈伤组织、烟草叶片中的瞬时表达情况。由于调节基因Ｃ１－Ｒ激活了植物体细胞内花色素苷的合成，因此不需任何生色底物，即可活体观察到花色素苷的表达。结果表明，对于目前仍主要通过基因枪法转化的几类主要粮食作物──小麦、玉米、水稻、枪击４８ｈ后，放大２倍便清晰可见红色斑点，且其表达强度远高于ＧＵＳ的表达，证明Ｃ１－Ｒ可作为一个很好的衡量打枪效果的指示，同时还证明其在双子叶植物──烟草叶片的基因枪转化瞬时表达体系中也起同样的作用。

In the present study, the transient expression of maize C1-R regulatory genes of anthocyanin biosynthesis in maize calli, wheat immature embroys, rice calli and tobacco leaves were described. C1-R regulatory genes could activate anthocyanin biosynthesis in vivo and the pigmented cells were visible. For major crops, including maize, wheat and rice, which were transformed mainly by biolistic method, C1-R genes were novel visible markers, and the expression was easier to detect than GUS gene. And these genes also worked well in transient expression in biolistic transformation system of tobacco leaves.