胃癌组织cagA阳性幽门螺杆菌感染与环氧化酶-2的关系

Analysis of cagA^+ Helicobacter pylori infection and expression level of COX-2 in gastric tumor tissue

目的观察胃癌组织中环氧化酶-2(COX-2)的表达情况,探讨cagA阳性幽门螺杆菌(Hp)感染与COX-2的关系。方法应用聚合酶链反应(PCR)检测42例胃癌患者胃癌组织、癌旁增生组织及癌旁正常胃黏膜组织中Hp cagA的表达情况。结果胃癌组织COX-2蛋白阳性率明显高于癌旁增生组织及正常胃黏膜组织,差异有统计学意义(P<0.01)。经PCR基因扩增42例胃癌标本中Hp cagA阳性25例,Hp cagA阴性17例。Hp cagA阳性组COX-2蛋白阳性表达率为88.0%(22/25),Hp cagA阴性组COX-2蛋白阳性表达率为35.3%(6/17),二者比较差异有统计学意义(P<0.01)。结论胃癌组织中存在COX-2的异常表达,cagA阳性Hp感染可能通过调节COX-2的表达水平促进胃癌的发生、发展。

Objective To observe the cyclooxygenase-2（COX-2） expression of gastric tumor tissue,and to study the relationship between cagA＋ Helicobacter pylori（Hp cagA＋ ） infection and COX-2. Methods 42 specimens of gastric cancer tissue （including gastric carcinoma, paracancerous hyperplastic tissue and paracancerous normal gastric mucosa） in our hospitals from January 2010 to August 2011 were collected and detected the Hp cagA expression by PCR. Results The COX-2 protein positive rate in the gas- tric tcancer tissue was significantly higher than that in the paracancerous hyperplastic tissue and normal gastric mucosa（P〈0.01）. Among 42 cases of gastric cancer specimens, Hp cagA＋ was in 25 cases, Hp cagA- was in 17 cases. The COX-2 protein positive rate was 88.0%（22/25） in the Hp cagA＋ group and 35.3%（6/17） in the Hp cagA- group. The COX-2 protein positive rate in the Hp cagA＋ gastric tissue was significantly higher than that in the Hp cagA- gastric tissue（P〈0.01）. Conclusion Abnormal ex- pression of COX-2 exists in gastric tcancer tissue, cagA＋ Helicobacter pylori infection may promote the occurrence and development of gastric cancer by regulating the expression level of COX-2.