摘要
用 4株小鼠肝炎病毒 (MHV1、MHV3、A5 9和 JHM)的混合液定量人工感染SCID小鼠、BAL B/ c裸小鼠、BAL B/ c小鼠 ,接种病毒后 1、2、5、8d分别取小鼠的全血、肝 ,用组织总 RNA抽提试剂盒提取总 RNA,用一步法 RT-PCR方法检测小鼠肝炎病毒。结果显示 :接种后 1 d,SCID小鼠和 BAL B/ c裸小鼠即可检测到小鼠肝炎病毒 ,而BAL B/ c小鼠至接种后 5 d才检测出小鼠肝炎病毒。
The SCID mice、BALB/c nude mice and BALB/c mice were intraperitoneally inoculated with quantitative mixture of MHV1, MHV3, A59, JHM and euthanized on 1st,2nd,5th,8th day after inoculation. The blood and liver samples were taken, and the liver was homogenized with plastic homogenizer in a 1.5ml microfuge tube for extracting total tissue RNA. Then, the RNA was transcribed into cDNA by using Mu MLV reverse transcriptase and the cDNA was amplified by PCR in same tubes . The results indicated that MHV of the blood and liver were detected on 1st day in SCID mice and BALB/c nude mice and on 5th day in BALB/c mice after inoculation.
出处
《上海实验动物科学》
2000年第3期141-143,共3页
Shanghai Laboratory Animal Science
基金
上海市科技发展基金!(974 1190 4 5)
