胃癌病变中p53和增殖细胞核抗原的表达及其与预后的关系

p53 and PCNA expression in gastric carcinoma and its relationship with prognosis

目的探讨抑癌基因p53和增殖细胞核抗原(PCNA)在胃癌和癌前病变中的表达及其与预后的关系。方法运用免疫组织化学方法检测肠上皮化生、中重度不典型增生、原发性胃癌组织中的p53和PCNA的表达情况,并设正常胃黏膜为对照组,观察p53和PCNA在正常胃黏膜→肠上皮化生→异型增生→胃癌的演变过程的表达。结果 p53蛋白在胃癌中表达显著高于异型增生,差异有统计学意义(P<0.01),并且与胃癌分化程度和淋巴结转移关系密切(P<0.05);PCNA在正常胃黏膜、肠上皮化生、异型增生和胃癌中表达逐渐递增,PCNA表达与胃癌分化程度、浸润深度以及淋巴结转移关系密切(P<0.05)。p53蛋白表达与PCNA标记指数呈正相关。结论 p53和PCNA在胃癌发生、发展中呈异常表达,检测p53和PCNA对判断胃癌临床预后有重要意义。

Objective To discuss the expression of p53 and proliferating cell nuclear antigen（PCNA） in gastric carcinoma and precancerous lesion and their relationship with prognosis. Methods P53 and PCNA expression in intestinal metaptasia,medium and severe atypical hyperplasia and primary gastric cancer tissues were measured by the immunohistochemical method. Meanwhile, the normal gastric mueosa were taken as the control group. The changes of p53 and PCNA expression in normal gastric mucosa,intesti- nal metaplasia,atypical hyperplasia and gastric cancer tissues were observed. Results p53 expression in gastric carcinoma was sig- nificantly higher than that in dysplasia（P〈0.01）, and closely related with gastric carcinoma differentiated degree and lymphatic metastasis（P〈0.05）. PCNA expression was progressively increased in normal gastric mucosa, intestinal metaplasia, atypical hyper- plasia and gastric cancer and closely related with gastric carcinoma differentiated degree,invasion depth and lymphatic metastasis（P 〈0.05）. There was positive correlation between p53 expression and PCNAL. Conclusion p53 and PCNA has abnormal expression in the occurrence and progression of gastric carcinoma. Detecting p53 and PCNA expression has important significance for predic- ting the clinical prognosis of gastric carcinoma.