妇科门诊就诊者HPV基因分型结果回顾分析

Gynecological Outpatients HPV Genotyping Results Review

目的:探讨女性HPV DNA检测在宫颈癌防治方面的意义。方法:应用PCR-反向点杂交技术对468例妇科门诊就诊者基因分型检测。结果:468例样本中,HPV感染者165例,阳性率35.25%,HPV感染人次233人次。检测高危型HPV(16,18,31,33,35,39,45,51,52,53,56,58,59,66,68,73,83)171人次,占感染总人次的73.39%;检出低危型HPV(6,11,42,43)62人次,占感染总人次26.61%。43例样本中包含了2～6种亚型的感染。结论:DNA杂交技术检测HPV基因分型,可一次检测多种亚型,有利于对HPV多重感染的诊断和宫颈癌的防治,可作为宫颈癌筛查的手段。

Objective ： To investigate the distribution of human papill omavirus （HPV） genotype infections and explore the application of HPV DNA test in the prevention and treatment of cervix cancer. Methods： 468 women were detected for HPV geno- typing by using DNA hybridization technique. Results： Of these 468 women, I65 women （35.25 % ） were found to be HPV positive. Among 233 cases of HPV infection 171 （73.39 % ）were infected with high risk HPV types （HPV16, 18, 31, 33, 35, 39, 45, 51,52, 53, 56, 58, 59, 66, 68, 73 and 83）, 62 （26.61% ） were with low risk types （HPV6, 11,42 and 43） among Chinese women. 2 - 6 subtypes were detected in 43 patients. Conclusion： DNA hybridization technique is useful for the diagnosis of multi HPV infection and therefore for the prevention and treatment of cervical cancer.