毛细管气相色谱法同时测定保妇康栓中冰片及3种倍半萜含量

Simultaneous determination of borneol and other three sesquiterpenes in Baofukang suppository by capillary GC

目的建立GC-FID含量测定方法,同时测定保妇康栓中冰片、呋喃二烯、莪术醇和莪术二酮的含量。方法采用HP-5石英毛细管色谱柱(30 m×0.25 mm,0.25μm)程序升温,流速为2.5 mL.min-1,分流比为10∶1,进样量为1μL。结果冰片、呋喃二烯、莪术醇和莪术二酮质量浓度分别在28.0～224.0(r=0.999 8)、16.0～128.0(r=0.999 9)、2.8～22.4(r=0.999 8)、4.0～32.0 mg.L-1(r=0.999 9)内线性关系良好,平均回收率分别为103.7%、91.6%、102.5%、99.5%,RSD分别为4.7%、3.6%、3.9%、4.3%(n=9)。结论本方法可为保妇康栓的质量控制提供科学依据。

Objective To establish a GC-FID method of Baofukang Suppository and develop this method for determination of borneol,furanodiene,curcumol and curdione at the same time.Methods A HP-5 quartz capillary column（30 m×0.25 mm,0.25 μm） programmed temperature was used at the flow rate of 2.5 mL · min-1 with split ratio of 10∶ 1;injection volume was 1 μL.Results Borneol,furanodiene,curcumol and curdione were linear in the range of 28.0-224.0（r=0.999 8）,16.0-128.0（r=0.999 9）,2.8-22.4（r=0.999 8）,4.0-32.0 mg · L-1（r=0.999 9）,the average recoveries were 103.7%,91.6%,102.5%,99.5%,RSD were 4.7%,3.6%,3.9%,4.3%（n=9）,respectively.Conclusions The validated method is simple and shows good accuracy and repeatability,which can provide scientific evidence for the quality control of Baofukang Suppository.