大豆根茬腐解产物的鉴定及化感作用的初步研究

Identification and allelopathy on the decomposition products from soybean stubs

采用 GC-MS分析法鉴定了培养试验获得的大豆根茬腐解 2周、4周产生的有机化合物的酸、碱性组分产物 ,并对腐解 2周、4周、8周各组分产物进行了化感作用的研究。结果表明 :大豆根茬腐解产物 (含微生物菌源根际土中的有机化合物 )十分丰富 ,有酸类、酯类、醇类、醛类、酚类、酮类、烃类等物质 ,其中有些有机化合物已被研究证明是化感物质 ,还有些未见报道 ;不同腐解时间产生的有机化合物有一定差异 ;对腐解产物进行生物检测试验 ,发现大豆根茬腐解 2周、4周、8周产生的有机化合物其酸性、碱性组分均抑制了大豆种子萌发和胚根生长 ;根茬腐解产物酸、碱性组分处理的胚根长与对照比较差异达显著或极显著水平 ,酸性组分的化感抑制作用大于碱性组分。此外 。

Acid and basic components of organic compounds from soybean stubs decomposed for two weeks and four weeks in culture experiments were identificated and the allelopathy of compounds from the decomposed stubs for two weeks,four weeks and eight weeks was studied by GC MS analysis.The results showed that the decomposition products from soybean stubs(including organic compounds in rhizosphere soil)were abundant,and they included organic acids,ester,alcohol,aldehydes,phenol,acetone and hydrocarbon.Some of them are allelochemicals and some organic compounds had not been documented as allelochemicals yet.There were definite differences in organic compounds from different decomposition time.Acid and basic components from soybean stubs decomposed for two weeks,four weeks and eight weeks all inhibited the germination of soybean seeds and radicle growth.The differences of radicle length were significant between the group treated with the components from decomposed soybean stubs and control group.Allelopathy inhibition of acid components is stronger than that of basic components.In addition,some actions of the decomposition products from soybean stubs in continuous cropping barriers were discussed.