检测肺结核早期患者血清miRNAs的临床意义

Significance of Serum miRNAs for early Detection of pulmonary tuberculosis

目的探讨肺结核早期患者检测血清潜在的生物标志miRNA(miRNAs)的临床意义。方法采集血清样本共60例,其中活动性肺结核患者及健康体检者各30例。用定量RT-PCR(qRT-PCR)检测血清miRNA的表达水平,并进行分析比较。结果肺结核患者血清miR-361-5p、miR-889及miR-576-3p水平显著增高,与健康对照者相比较,差异有统计学意义(P<0.05)。结论血清miR-361-5p、miR-889和miR-576-3p可作为肺结核早期诊断的潜在标志物。

Objective To investigate the potential biomarkers of pulmonary tuberculosis （TB） in clinical significance. Meth- otis Totally,60 samples included 30 pationts with active tuberculosis and 30 healthy controls respectively. Using quantitative reverse transcriptase polymerase chain reaction （qRT-PCR） to detect samples＇miRNAs and analyze data. Results Serum miR-361-5p,miR- 889 and miR-576-3p of TB group were significantly higher than health group（P 〈 0. 05）. Conclusions Serum miR-361-5p,miR- 889 and miR-576-3p levels can be used as promising biochemical markers for the early diagnosis of TB.