脑梗死患者CD40—1C／T基因多态性与血清sCD40L的相关性

Correlation between CD40 -1C/T gene polymorphism and serum sCD40L

目的探讨脑梗死患者CIM0—1C／T基因多态性对血清游离CD40L（sCD40L）表达的影响。方法选取急性大动脉粥样硬化性脑梗死患者209例作为病例组，选取同期无卒中病史体检者87例作为对照组。采用聚合酶链反应．限制性片段长度多态性（PCR—RFLP）技术检测CD40基因多态性并测序鉴定，采用双抗体夹心酶联免疫吸附法（ELISA）检测血清sCD40L浓度。结果病例组CC基因型（31．6％）及C等位基因频率（53．8％）显著高于对照组（17．2％，39．1％），差异有统计学意义（x2=6．94，10．69，P〈0．01）；病例组血清sCD40L表达水平显著高于对照组[（3．97±1．20）ng／mlV8（2．69±0．88）ng／ml]，差异有统计学意义（t=10．19，P〈0．01）。病例组血清sCIMOL表达水平在CC[（4．55±1．16）ng／ml]、CT[（3．93±1．17）ng／m1]、Tr[（3．27±0．90）ng／ml]各基因型间差异有统计学意义（F=19．22，P〈0．01），CC基因型患者血清sCD4OL水平明显高于CT和TT基因型者，CT基因型患者血清sCIMOL水平明显高于，ITll基因型者；对照组血清sCD40L表达水平在CC[（2．91±0．79）ng／m1]、CT[（2．674-0．89）ng／m1]、Tr[（2．614-0．91）ng／m1]各基因型间差异无统计学意义（F=0．619，P=0．541）。结论脑梗死患者CD40—1C／T基因多态性与血清sCD40L的表达水平有关，CC基因型血清sCD40L水平明显增高。

Objective To investigate the correlation between CD40 - 1C/T gene polymorphism and serum soluble CIMOL （sCIMOL） expression in cerebral infarction. Methods According to the inclusion and exclusion criteria, select the acute large artery atherosclerosis in patients with cerebral infarction as the case group, select the same period without a history of stroke examination subjects as the control group. Pol- ymerase chain reaction - restriction fragment length polymorphism （PCR-RFLP） technology was used to de- tect CD40 gene polymorphism and sequencing, double-antibody sandwich enzyme-linked immunosorbent as- say （ELISA） was used to detect the expression levels of serum sCD40L. Results A total of 209 patients with large artery atherosclerotic cerebral infarction （case group） and 87 subjects without history of stroke （control group） were included. The CC genotype （31.6%） and C allele frequency （53.8%） of the case group were significantly higher than that of the control group （ 17.2%, 39. 1% ） （ x2 = 6. 94,10. 69, P 〈 0. 01 ）. Case group serum sCD40L expression level was significantly higher than those in the control group [ （3.97 ± 1.20） vs （2. 69 ± 0. 88） ] （ t = 10. 19, P 〈 0. 001 ）. Case group serum sCIMOL expression lev- el was different among CC, CT, and TT genotypes （ F = 19. 22, P 〈0. 001 ）, serum sCD40L level （4. 55 ± 1.16） of CC genotype in patients was significantly higher than that of CT （3.93± 1.17） and Tr geno- types （3.27± 0. 90）, serum sCD40L level （3.93 ± 1.17） of CT genotype in patients was significantly higher than the TY genotype （3.27 ±0. 90）. The serum sCD40L expression level of the control group in the CC, CT, TY has no statistically significant differences among various genotypes [ （2.91 ±0. 79）, （2. 67 ±0. 89）, （2.61 ±0. 91 ）, F =0. 619, P =0. 541）. Conclusions CD40 - 1C/T gene polymorphism is as- sociated with serum sCD40L expression level in cerebral infarction, serum sCD40L level of the CC genotype was significantly higher.