凉血愈肠汤对大鼠放射性肠炎肠黏膜的修复机制

Evaluation the regeneration effect of Liang-Xue-Yu-Chang decoction on intestinal epithelium of rats with radiation enteritis

目的 探讨凉血愈肠汤灌胃治疗大鼠放射性肠炎的作用机制.方法 随机数字表法将60只雄性SD大鼠分为3组,正常对照组、蒸馏水组（10 Gy剂量照射后给予蒸馏水灌胃）和凉血愈肠汤组（10 Gy剂量照射后给予凉血愈肠汤灌胃）,每组20只.连续7d分别给予照射后的大鼠蒸馏水和凉血愈肠汤灌胃,在实验结束前经腹腔注入5-溴脱氧尿苷（BrdU）,采用SP方法及光镜观察肠腺上皮细胞增殖核抗原（PCNA）、Ki67和BrdU标记阳性细胞.结果 凉血愈肠汤组大鼠的肠上皮细胞PCNA、Ki67和BrdU标记阳性细胞数（22.0±2.7、71.2±5.7、26.2±5.9）显著多于蒸馏水组模型大鼠（11.2±1.9、61.6±5.5、11.3±2.2）（t=14.629、5.420、11.582,P＜0.05）,少于正常对照组大鼠（30.4±5.7、86.6±5.1、32.3±3.2）（t=14.291、9.004、4.731,P＜0.05）.结论 凉血愈肠汤可促进急性放射性肠炎大鼠肠上皮细胞的增殖.

Objective To study the effects of Liang-Xue-Yu-Chang decoction on intestinal epithelium of rats with radiation enteritis.Methods Sixty male SD rats were divided into three groups randomly：normal rats as control,abdominal radiation control group given distilled water after 10 Gy radiation,and abdominal radiation plus drug group given Liang-Xue-Yu-Chang decoction after 10 Gy radiation.Drugs or distilled water were chronically administered to animals for 7 days.After that,5-bromodexyridine （BrdU） was injected into abdominal cavity,SP immunohistochemistry was used to evaluate intestinal epithelium proliferating cell nuclear antigen （PCNA） and the positive cells labeled with Ki67 and BrdU.Results The number of intestinal epithelium cells with PCNA expression,positive Ki67 and BrdU were 22.0 ＋ 2.7,71.2 ＋ 5.7,and 26.2 ＋ 5.9 respectively in the drug treatment group,significantly higher than those in the abdominal radiation control group （11.2 ＋ 1.9,61.6 ± 5.5,and 11.3 ± 2.2） （t =14.629,5.420,11.582,P 〈 0.05）,but lower than those in the normal control group （30.4±5.7,86.6 ±5.1,and 32.3 ±3.2）（t =14.291,9.004,4.731,P〈0.05）.Conclusion Liang-Xue-Yu-Chang decoction could improve the proliferation of intestinal epithelium in the rats with radiation enteritis.