PSP94融合蛋白在大肠杆菌中的表达及其抗前列腺癌活性分析

Expression of Human Prostate Secretory Protein of 94 Amino Acids Fusion Protein in E.coli and Activity Assay of the Expressed Product

在从成年人正常前列腺组织中获得人９４ 个氨基酸的前列腺分泌蛋白（ＰＳＰ９４）ｃＤＮＡ 基础上，利用ＰＬ 表达系统，实现了人ＰＳＰ９４ 成熟肽Ｎ末端带有１９ 个外源氨基酸的融合蛋白在大肠杆菌中的表达。目的蛋白在细胞中主要以包涵体形式存在，表达量约占菌体总蛋白的３０ ％ ，分子量约为１６－５ｋＤ。表达产物在人前列腺癌细胞ＰＣ３ 上活性分析表明，该融合蛋白能明显抑制前列腺癌细胞的生长。

We had previously cloned the whole length cDNA of human prostate secretory protein of 94 amino acids (PSP94) from normal adult prostatic tissues by RT\|PCR.In this study, we reported expression of human PSP94 fusion protein in E.coli.The chimeric gene coding for 19aa\|PSP94 fusion proteins under control of the P\-L promoter was constructed. The expression was assayed by SDS\|PAGE,which showed that the molecular weight of the 19aa\|PSP94 fusion protein was in accordance with the estimated value of 16.5kD.It was 30% of total bacterial protein and in the form of inclusion body. Through biological activity analysis, 19aa\|PSP94 fusion protein has anti\|prostatic cancer activity.\;