重型乙型肝炎患者T细胞受体BVCDR3家族表达水平分析

The study of the expression of TCR BV CDR3 family in fulminant hepatitis B patients

目的探讨重型乙型肝炎（FHB）患者外周血T淋巴细胞受体（TCR）B链V区（BV）互补决定区3（CDR3）基因各家族谱系的表达及其临床意义。方法选择我院2010年10月至2012年3月收治的FHB患者（FHB组）28例及20例健康体检者（健康对照组），分别采集其外周血，肝素钠抗凝，分离单个核细胞，提取RNA并应用RT-PCR方法扩增TCRBV的CDR3区基因24个家族，并进行两组间扩增产物定量分析的比较。结果FHB组ACtl，ACtl2，△ct20含量较健康人群增多（P〈0．05）；△ct5、ACff、ACtl3、ACtl4、ACtl5、△ct22、ACt23含量较健康对照组下降（P〈0．05）。结论FHB患者TCRBVCDR3区基因部分家族表达水平改变，提示T淋巴细胞免疫应答可能参与了FHB的发病过程。

Objective To study the expression of TCR BV CDR3 family in fulminant hepatitis B （FHB） patients. Methods Totally 28 patients with fulminant hepatitis B （FHB） （ FHB group）, who were treated in our hospital from Oct. 2010. to Mar. 2012 ,and 20 healthy controls（ HC group） were included in the study. PBMCs were isolated from antieogulatcd blood,and RT-PCR was used to detect the levels of TCR BV CDR3 family in the 2 groups. Results The levels of ACt1, ACtl2 and ACt20 in FHB group were higher than those in HC group （P 〈 0. 05 ） ; The levels of ACTS, ACt＇/, ACt13, ACt14, ACt15, ACt22, ACt23 in FHB group were lower than those in HC group （P 〈 0. 05 ）. Conclusions The result indicates that cellular immunology is involved in the pathogenesis of the liver inflammation process of FHB.