两种酶联免疫法检测丙型肝炎病毒抗体结果分析

Comparison of two kinds of enzyme-linked immunosorbnent assay for detection of anti-hepatitis C virus

目的比较双抗体夹心酶联免疫法和间接酶联免疫法检测丙型肝炎病毒抗体（抗-HCV）时的灵敏度和特异性的差异。方法选择一种夹心法试剂检测51份一种或两种间接法试剂阳性献血标本，并采用免疫印迹和核酸检测作为确证试剂。结果与确证试剂相比，两种间接法试剂和一种夹心法试剂的假阳率分别为40．9％，59．1％和2．2％，阳性检出率均为100％，但夹心法的分析灵敏度比间接法高2～6倍。结论夹心法试剂在灵敏度和特异性上都显著优于间接法试剂。

Objective To compare the difference of sensitivity and specificity on the results of the same samples determined by indirect ELISA and sandwich ELISA. Methods A 51 anti-HCV positive serum samples obtained from donors were screened by two indirect ELISA kits initially, and then were detected using one sandwich ELISA kit, and were confirmed by recombinent immunoblot assay （RIBA） and HCV- RNA. Results To compared with HCV-RNA, false positive rate of two kinds of indirect ELISA and one sandwich ELISA was 40. 9% , 59.1% and 2. 2% respectively. The positive rates of them were 100% , but the analytical sensitivity of sandwich ELISA was more than indirect ELISA 2 to 6 times. Conclusions The sensitivity and specificity of sandwich ELISA was significantly better than those of indirect ELISA.