阿瓦斯丁对HaCaT细胞体外增殖及其VEGF表达的影响

Effect of Avastin on the Proliferation and the Expression of VEGF in Cultured HaCaT Cells

目的探讨阿瓦斯丁对HaCaT细胞体外增殖及VEGF表达的影响。方法体外培养HaCaT细胞,不同浓度阿瓦斯丁分别作用24h,48h和72h后,CCK-8法检测阿瓦斯丁对HaCaT细胞体外增殖的抑制作用;Western印迹和RT-PCR法检测不同浓度阿瓦斯丁作用HaCaT细胞48h后对HaCaT细胞VEGF蛋白及mRNA表达的影响。结果不同浓度阿瓦斯丁对HaCaT细胞的体外增殖均具有抑制作用,且这种抑制作用具有剂量依赖性,与对照组相比差异有统计学意义(P<0.05);不同浓度阿瓦斯丁作用HaCaT细胞48h后,VEGF蛋白及mRNA的表达下调,与对照组相比差异有统计学意义(P<0.05)。结论阿瓦斯丁可抑制HaCaT细胞的体外增殖并且能抑制VEGF蛋白及mRNA的表达。

Objective To investigate the effects of Avastin on proliferation and VEGF expression in cultured HaCaT cells. Methods Cultured HaCaT cells were treated with different concentrations of Avastin. The changes of cell proliferation were determined by CCK-8 assay at 24h,48h and 72h after the treatment. RT-PCR and Western blot were used to detect the expression of VEGF mRNA and protein at 48h after the treatment. Results Avastin at all tested concentrations inhibited HaCaT cell proliferation in dose dependent manner （P 〈 0.05）. By 24h after treatment avastin at all tested concentrations significantly lowered the expression levels of VEGF protein and mRNA as compared to vehicle（P 〈 0.05）. Conclusion Avastin inhibits HaCaT cell proliferation, and the expression of VEGF protein and mRNA.