腺苷酸活化蛋白激酶在卵巢肿瘤中的表达及临床意义

Expression and significance of AMP-activated protein kinase in ovarian tumor

目的探讨腺苷酸活化蛋白激酶(AMPK)在卵巢肿瘤组织中的表达及临床意义。方法收集手术标本存档蜡块56份。其中,正常卵巢组织6份,卵巢良性上皮性肿瘤组织20份,卵巢癌组织30份。用免疫组化比较各组蜡块标本AMPKα1、AMPKα2及p-AMPK蛋白表达。结果AMPKα1蛋白表达于细胞的胞浆;卵巢癌组织中AMPKα1蛋白的阳性表达率为30%,与卵巢良性肿瘤组织的35%和正常卵巢组织的33%相仿(P>0.05)。AMPKα2蛋白也表达于胞浆,少量表达于胞核;其在卵巢癌中的阳性表达率为80%,明显高于卵巢良性肿瘤组织的45%和正常卵巢组织的33%(P<0.05)。卵巢癌中p-AMPK蛋白表达明显低于卵巢良性肿瘤组织和正常卵巢组织(P<0.05)。AMPKα1和AMPKα2蛋白的表达与不同临床病理指标之间无明显相关性。结论卵巢癌组织中AMPKα2蛋白高表达,表明AMPK信号传导途径在卵巢癌发病机制中有重要作用。

Objective To investigate the expression and significance of AMP-activated protein kinase in ovarian tumors. Methods Immunohistochemical staining was used to examine the expressions of AMPKal, AMPKa2 and p-AMPK in 30 patients with ovarian carcinoma（group A）, 20 cases with ovarian cystadenoma （group B） and 6 normal ovarian tissue samples （group C）. Results The AMPKal and AMPKa2 were expressed in the cytoplasm and AN/PKa2 was expressed in the nucleus in a small amount as well. The positive expression rate of AMPKal protein in group A was 30% ,which was similar to 35% in group B and 33% in group C（P〉0. 05）. The positive expression rate of AMPKa2 protein in group A was 80%, which was significantly higher than 45% in group B and 33% in group C（P〈0. 05）. The positive expression of p-AMPK in group A was lower than that in groups of B and C （P〈0. 05）. The positive expressions of AMPKal and AMPKa2 were not correlated to the clinicopathological parameters. Condution AMPKa2 is overexpressed in ovarian cancer, suggesting that AMPK signal pathway plays an important role in the development of ovarian cancer.