莪术油对人肺腺癌A549细胞增殖和凋亡的影响

ZTO Inhibited Proliferation and Induced Apoptosis of Human Lung Adenocarcinoma Cell Line A549

该研究旨在探讨莪术油(zedoray turmeric oil,ZTO)对人肺腺癌A549细胞增殖的影响及诱导细胞凋亡作用。不同浓度莪术油作用A549细胞48 h后,采用MTT法检测细胞存活率;光学显微镜、荧光显微镜和透射电镜观察细胞形态结构;流式细胞术检测细胞周期、细胞凋亡率和线粒体膜电位(ΔΨm);Real Time RT-PCR和Western blot检测Bcl-2/Bax表达水平。结果显示,莪术油对A549细胞生长具有剂量依赖性抑制作用,莪术油作用A549细胞48 h的最佳浓度是80μg/mL,增殖抑制率为(77.462%±0.681%),显微镜下观察细胞呈明显凋亡现象,细胞凋亡率为(27.31%±0.43%),ΔΨm显著下降(P<0.01),细胞阻滞于S期和G2期;Bcl-2的表达下调,Bax的表达明显增加,Bcl-2/Bax比值显著降低(P<0.01)。提示莪术油能抑制肺腺癌A549细胞增殖,通过上调Bax下调Bcl-2诱导其凋亡。

The study investigated the effects of zedoray turmeric oil （ZTO） on the proliferation and apoptosis of human lung adenocarcinoma cell line A549. The inhibitory effect of ZTO on the growth ofA549 cells was tested by MTT method in vitro. Light microscope, fluorescence microscope and TEM were used to observe the morphology change of apoptosis in A549 cells. The apoptosis, mitochondrial membrane potential （△ψm） and cell cycle distribution of A549 cells were assayed by flow cytometry. The levels of Bcl-2/Bax were measured by Real Time RT-PCR and Western blot. The results showed that ZTO inhibited the growth ofA549 cells in a dose-dependent manner, consistent with the results of cell morphology. After exposure to 80 ~tg/mL ZTO for 48 h, cell apoptosis was highest, the level of △ψm decreased and caused a significant S arrest at the expense of G1 and G2-M phase cell numbers. ZTO also reduced the ratio of Bcl-2/Bax. The results suggested that ZTO depresses the cell growth and induces the apoptosis of human lung adenocarcinoma cell line A549 through up-regulation of Bax and down-regulation of Bc 1-2.