培养基清除结皮对蛹虫草原基形成的影响

Effects of medium callus on Cordyceps militaris primordium formation

为探讨培养基清除结皮对蛹虫草原基的形成影响,提高食用真菌蛹虫草的培养产量和品质,在无菌条件下,用无菌刀切除长满菌丝的培养基上表面部分,在温差和光周期刺激下培养,考察菌丝扭结和子实体生长情况,并检测蛹虫草子实体虫草素、虫草酸和多糖含量。结果显示培养基清除结皮组蛹虫草子实体生长快、颜色较深、易形成孢子头,其虫草素和腺苷含量显著升高(P<0.05),但虫草多糖含量变化不显著(P>0.05)。培养基清除结皮可显著地促进蛹虫草原基的形成,提高子实体产量和品质。

To study the influences of medium callus on the formation of Cordyceps militaris primordium, the callose surface part of the culture was removed with a sterile knife on the sterile conditions. After removing the surface callus, the culture was incubated under the stimulation of temperature difference and photoperiod in order to further investigate the growth states of mycelium kinks and fruiting bodies, and the content changes of cordycepin, cordyceps acid and polysaccharide. Our results showed that after removing the callus the culture demonstrated significantly faster growth, darker color, more easily forming the spore head and increasing contents of cordycepin and adenosine as compared with the control tested group （P〈0.05）. It is concluded that the formation of Cordyceps militaris primordium could be significantly enhanced by removing the culture callus, resulting in the improvement of the yield and quality of mycelium kinks and fruiting bodies.