双孢蘑菇子实体发育后期差异表达蛋白质分析

Analysis of differentially expressed proteins in later developing stage fruitbody of Agaricus bisporus

为探讨双孢蘑菇子实体发育后期的蛋白质表达变化,对双孢蘑菇As2796子实体采收期、成熟期和开伞期的蛋白质组进行了双向电泳(2-DE)分析,发现了16个表达差异明显的蛋白质。通过质谱分析(MALDI-TOF/TOF MS)和数据库检索,有14个差异蛋白质获得鉴定。其中磷酸烯醇式丙酮酸水合酶与能量代谢相关,T-蛋白复合体1、蛋白酶体、5-甲基四氢三谷氨酸-同型半胱氨酸甲基转移酶、1-吡咯琳-5-羧酸脱氢酶、精氨酸酶与氨基酸或蛋白质代谢直接相关,而GTP结合蛋白则参与细胞的多种生命活动,在细胞的生长发育过程中起着重要的作用。另外7个为功能未知的蛋白质。

To discuss the protein expression changes in later developing fruitbody of Agaricus bisporus, the technique of two-dimensional electrophoresis （2-DE） was used to analyze the protein expression differences among the harvesting, maturity and opening stage of A. bisporus As2796 fruitbody. As a result, sixteen proteins were found to be differentially expressed. Based on the results of MALDI-TOF/TOF MS analysis and database searching, fourteen proteins were identified successfully. Among them phosphopyruvate hydratase is related to energy metabolism, and T-complex protein 1, proteasome, 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase, 1-pyrroline-5-carboxylate dehydrogenase, arginase are all directly related to amino acid or protein metabolism, while GTP-binding protein is related to several intracellular bioprocess, and play important roles in cellular growth, development, differentiation etc. Seven others were unknown proteins.