不同运动方式对兔膝关节骨性关节炎关节液中TNF-α和MMP-1含量的影响

Effect of Different Movements on the Level of TNF-αand MMP-1 in Joint Fluid of Rabbit with Knee Osteoarthritis

目的:观察不同运动方式对兔膝关节骨性关节炎(OA)关节液中肿瘤坏死因子-α(TNF-α)和基质金属蛋白酶-1(MMP-1)水平的影响。方法:采用新西兰大耳兔左后膝伸直位管型石膏固定8周的方法建立OA动物模型,通过施加主动活动、被动活动、自由活动等运动方式,检测各组兔膝关节液中TNF-α和MMP-1含量。结果:自由活动组、主动活动组、被动活动组兔膝关节液中TNF-α和MMP-1含量均明显低于模型组(P<0.05或P<0.01)。结论:适宜运动能降低兔膝关节骨性关节炎关节液中TNF-α和MMP-1水平,延缓病情发展。

Objective：To observe the effect of different movements on tumor necrosis factor-alpha （TNF-a） and matrix metalloproteinase-1 （MMP-1） in rabbit with knee osteoarthritis （OA）. Methods： Animal models of OA were conducted by fix- ing the left posterior knee joint in extension using plaster cast for 8 weeks in New Zealand rabbits. After imposing active movement, passive movement and free movement on the knee joint, the levels of TNF-α, MMP-1 from synovial fluid in rabbit with osteoarthritis were measured. Resuits：The levels of TNF-α and MMP-1 in synovial fluid in movement groups were lower than those in the model group （P〈0.05 or P〈0.01）. Conclusion：Proper movement can decrease the levels of TNF-α and MMP-1 from synovial fluid in rabbit and delays the osteoarthritis exacerbation.