白藜芦醇对戊四氮致痫大鼠脑脊液、血清S100B蛋白的影响

Effects of Resveratrol on the levels of S100B protein in cerebrospinal fluid and serum of pentylenetetrazole-kindled rats

目的研究白藜芦醇(Res)对戊四氮致痫大鼠脑脊液、血清S100B蛋白的影响。方法采用戊四氮(PTZ)腹腔注射建立慢性癫痫模型,造模成功后予以Res(15 mg/kg.d)灌胃干预10 d;采用酶联免疫吸附法测定脑脊液、血清S100B蛋白含量,海马标本行Nissl染色。结果经28 d连续给药,18只大鼠符合Racine点燃标准,Res干预组大鼠痫性发作潜伏期明显延长,且发作时间明显低于癫痫模型组、二甲基亚砜组(P<0.05)。海马Nissl染色提示Res干预对大鼠海马CA1、CA3区神经元有保护作用(P<0.05),而对齿状回保护作用不明显(P>0.05)。Res干预组大鼠脑脊液、血清S100B蛋白含量低于癫痫模型组、二甲基亚砜组(P<0.05)。结论 Res降低PTZ致痫大鼠脑脊液、血清S100B蛋白含量,或许减缓癫痫发作脑损伤发挥神经保护作用。

Objective To study effects d resveratrol on SI（1）B protein in cerebrospinal fluid and serum of pentylenetetrazole （PIZ） kindled rats. Methods By injected intraperitoneally pentylenetetrazole chronic FIE-kindling rrtxtel was established. The resveratrol （Res） was applied intragastrically at a dose of 15 mg/kg once a day for 10 d. Enzyme-linked immunosorbent assay was used to evaluate the levels of S100B protein in cerebrospinal fluid and serunr Hippocampus specimen Nissl staining was used to evaluate the degenerating neurons. Results With a continuous dosing for 28 d, 18 rats reached the Racine kindling standard, qhe seizures latent period in Res group was significantly prolonged, and its duration was significantly lower than those of the epilepsy n-txtel goup and the dimehylsulfoxide （DMSO） group （P 〈0. 05）. Nissl staining indicated Res intervention protected against PTZ-induced death of neuronal cells in CA1 as well as CA3 regions （P 〈0.05）, but not in dentate gyms （P 〉0.0fi）. rlhe levels of S100B protein in cerebrospinal fluid and serum in Res intervention gonp were lower than those of epilepsy rrtxtel group and DMSO group （P 〈0. 05）. Conclusion Res reduces the Sll30B protein level in cerebrospinal fluid and serum of PIZ-induced seizure rats, which might play a neuroprotective role by alleviating the seizure-induced brain injury.