摘要
玉米 (ZeamaysL .)种子在萌发期间热激处理 (42℃ )时蛋白质合成率低于对照 (2 5℃ ) ;高活力种子胚热激蛋白的合成率最高。在 42℃热激处理时玉米种子胚中合成的热激蛋白的分子量分别为 73、6 5、6 2、5 4、18kD等 5种。高活力种胚合成的热激蛋白在量上高于低活力种子 ,高活力种胚合成的特异性热激蛋白 5 6kD可以作为衡量种子活力的指标。双向电泳表明高低活力种子间热激蛋白的合成有更多质上的差异。
The synthetic rate of proteins was lower at high temperature stress (42 ℃) than that at normal condition(25 ℃). Seeds with high vigor synthesized more heat shock proteins (HSPs) during germination. Five kinds of HSPs were found at 42 ℃ in maize (Zea mays L.) seeds, and their molecular weight was 73 kD, 65 kD, 62 kD, 54 kD and 18 kD respectively. Quantitative difference was found between seeds with high and low vigor. Seeds with high vigor had a special HSP of 56 kD, this might be used as an index of seed vigor. 2D_PAGE showed more qualitative difference between seeds with high and low vigor for the synthesis of HSPs.
基金
广东省自然科学基金
教育部留学回国人员启动基金
关键词
玉米
种子活力
萌发
热激蛋白
maize
seed vigor
germination
heat shock proteins
