外植体和植物生长调节剂对盾叶薯蓣愈伤组织诱导的影响

Effects of Different Explants and Plant Growth Regulators on Callus Induction of Dioscorea zingiberensis

以盾叶薯蓣(Dioscorea zingiberensis C.H.Wright)茎段和叶片为外植体,研究了不同植物生长调节剂对盾叶薯蓣愈伤组织诱导的影响。结果表明,茎段作外植体更有利于盾叶薯蓣愈伤组织的诱导,最佳的愈伤组织诱导培养基为MS+1.5 mg/L 2,4-D+1.0 mg/L 6-BA,诱导30 d时愈伤组织诱导率达100%,愈伤组织为浅黄色、质地疏松、颗粒状;以叶片为外植体,愈伤组织诱导培养基中添加1.0 mg/L 2,4-D+0.5 mg/L 6-BA时诱导率最高,为93.75%。盾叶薯蓣愈伤组织的生长曲线呈"S"型,0～12 d为延迟生长期,增重缓慢;12～30 d为指数生长期,生长旺盛,增殖迅速;30～36 d为缓慢生长期,因此诱导的愈伤组织需在接种后30 d内继代培养。

Using stems and leaves as explants, the effects of different plant growth regulators on callus induction of Dioscorea zingiberensis C. H. Wright were studied. The results indicated that stems were better explants for callus induction than leaves. The optimum callus induction medium for stems was MS＋1.5 mg/L 2,4-D＋1.0mg/L 6-BA, as the induction rate could reach up to 100%. The callus was light yellow, loose and graininess. Using leaves as explants, the optimum callus induction medium was MS＋1.. mg/L 2,4-D ＋0.5 mg/L 6-BA, as the induction rate was 93.75%. Growth curve of callus was S-like and could be divided into three phases, lag growth phase（0-12d）, log growth phase（12-30 d） and slow growth phase （30- 36 d）, thus the subculture of callus should be carried out within 30 d after inoculation.