叶绿体DNA片段的RFLP分析在黄精族系统学研究中的应用

Molecular systematic studies on the tribe Polygonateae (s.l.) in China based on RFLPs data of PCR-amplified chloroplast DNA fragments

对广义百合科黄精族 6属 2 3种及铃兰族 1属 1种的叶绿体基因组trnK和rpl1 6两个基因片段进行了PCR RFLP分析 ,结果表明 :trnK基因的PCR产物在各类群间几乎不存在长度变异 ,均约 2 60 0bp ,而rpl1 6基因则在各属之间及黄精属内表现出长度变异 ,变异范围在 1 1 40～ 1 32 0bp之间 ;限制性酶切位点的同源性分析显示 ,黄精属、竹根七属、鹿药属和舞鹤草属构成的狭义黄精族与铃兰族中的铃兰属有较近的亲缘关系 ,并支持将扭柄花属和万寿竹属从广义百合科黄精族中分出的观点 ;在狭义黄精族内 ,黄精属与竹根七属聚成一支 ,鹿药属与舞鹤草属聚成另一支 ,为探讨族内属间的系统演化关系提供了分子生物学方面的证据。另外 ,本研究结果支持将金佛山黄精从鹿药属转隶至黄精属的观点。

WT5BZ]Phylogenetic relationships in the tribe Polygonateae s.l. of Liliaceae were investigated with an RFLP analysis of two PCR amplified chloroplast genome DNA fragments. One fragment is the transfer RNA gene for lysine(trnK gene) including the maturase encoding gene matK, and the other is the rpl16 gene which codes for a chloroplast ribosomal protein in large subunit. The trnK gene is ca. 2600 bp in length in all the taxa, but the rpl16 gene ranges from ca. 1140 bp to ca. 1320 bp in length among different genera and in Polygonatum. The results suggested that the tribe Polygonateae s. str. consisting of Polygonatum, Disporopsis, Smilacina and Maianthemum is closely related to Convallaria in Convallarieae, and supported the earlier results that Streptopus and Disporum should be removed out from the tribe Polygonateae s.l.. The results also showed that Polygonatum formed a clad with Disporopsis, while Smilacina lumped with Maianthemum in the tribe Polygonateae s. str.. In addition, the present study supports the view that S. ginfoshanicum should be transferred from Smilacina to Polygonatum. [WT5HZ]