miR-126在肺癌中的表达及其与VEGF的相关性

The Expression ofmiR-126 in Lung Cancer and Its Correlation with VEGF

目的：研究miR-126在肺癌中与血管内皮生长因子（VEGF）蛋白表达的相关性，探讨miR-126与肺癌的作用机制。方法：采用组织芯片平台，应用原位杂交方法（IsHH）检测56例肺癌组织、41例相应的癌旁组织和15例正常肺组织的miR-126表达；免疫蛋白印迹法（WestemBlotting）检测VEGF蛋白的表达。结果：肺癌组织miR-126阳性表达率均显著低于癌旁组织和正常肺组织（P〈0．05）；vEGF蛋白在肺癌组织中的表达率均显著高于癌旁组织和正常肺组织（P〈0．05）。肺癌组织中miR-126的低表达与肿瘤的淋巴转移密切相关，与病理分级有一定关系。相关性检验表明miR．126与VEGF表达呈显著负相关（P〈0．05）。结论：miR-126可能作为肺癌重要的抑癌基因及其转移的潜在标志。

Objective： To study the expression correlation in miR-126 and protein of vascular endothelial growth factor （VEGF） within lung cancer, and to explore the action mechanisms of miR-126 and lung cancer. Methods： Using tissue microarray platform, miR-126 expressed in lung cance： tis- sues of 56 cases and corresponding paracancerous tissues of 41 cases, and normal lung tissue of 15 cases were examined, applied with situ hybridiza- tion （ISHH）; to detect the expression of VEGF protein with western blotting. Results： The positive expression rate of lung cancer with miR-126 was significantly lower than that of paracancerous tissues and normal lung tissues （P〈0.05）; the expression rate of VEGF protein in lung cancer tissues was significantly higher than that in paracancerous tissues and normal lung tissiaes （P〈0.05）. The low expression of miR-126 in lung cancer tissues was closely related with lymphatic metastasis of tumor, where was a certain relationship with the pathological grading. Correlation tests showed that miR-126 and VEGF expression were significantly negative correlation （P〈0.05）. Conclusion： miR-126 can be used as an important tumor suppressor gene of lung cancer, and potential mark of its metastasis.