三核苷酸重复及相关序列宏观弯曲的预测

THE THEORETICAL PREDICTION OF MACROSCOPIC CURVATURE OFTRIPLET REPEATS AND OTHER RELATED SEQUENCES

给出描述36个四联体内碱基对平面间相互方位和碱基对间沿长轴方向位移的参数集。考虑到碱基对梯阶间的非紧邻相互作用 ,提出DNA宏观弯曲的经验预测规则。给出DNA宏观弯曲的定义 ,使用联系碱基对梯阶间的变换矩阵计算宏观弯曲。对18个与人类遗传病有关的三核苷酸重复及相关序列预测了宏观弯曲。理论结果和相应的实验数据作了比较 ,对结果的理论含义作了讨论。

The angular parameters (Ω,ρ and τ) describing the orientation of successive base-pair planes and the translation parameters (Dy) along the long axis of base-pair step have been calculated for thirty-six tetramers, which affords the basis of the predicting the macroscopic curvatures of DNA. Empirical rules, which take into account the non-neighbor interactions between the base-pair steps, have been presented to predict DNA curvature. A definition of DNA curvature has been made. The transformation matrix involving the angular parameters relating successive base-pair steps has been used to investigate the DNA curvature. The macroscopic curvature of triplet repeats sequences associated with human genetic disease and other related sequences have been predicted. The theoretical results have been compared with the corresponding experimental data. The theoretical implications have been discussed.